Leland Stanford Junior University

Screen Holdings Co., Ltd.

Ryosuke Itoh

Olav Solgaard

Michael J Mandella

Joseph Russell Landry

Stephen Sanborn Hamann

A system, including a structured illumination stage to provide a spatially modulated imaging field is provided. The system further includes a spatial frequency modulation stage to adjust the frequency of the spatially modulated imaging field, a sample interface stage to direct the spatially modulated imaging field to a sample, and a sensor configured to receive a plurality of fluorescence emission signals from the sample. The system also includes a processor configured to reduce a sample scattering signal and to provide a fluorescence emission signal from a portion of the sample including the spatially modulated imaging field. A method for using the above system to form an image of the sample is also provided.

Structured illumination in inverted light sheet microscopy

Improved image quality by structured illumination or pivoting illumination and faster image acquisition are both achieved. A line light enters first to fifth virtual pixels of a grating light valve, and first to fifth lights are emitted respectively from the first to fifth virtual pixels. The intensities and phases of the first to fifth 0th-order lights respectively depend on the arrangements of sub-pixels included in the first to fifth virtual pixels. The first to n-th 0th-order lights are extracted respectively from the first to n-th lights, and the first to n-th 0th-order lights are converted respectively into first to fifth light sheets. The first to fifth light sheets are created at a portion to be illuminated. The arrangements of the sub-pixels included in the first to fifth pixels are controlled such that a structured light sheet or pivoting light sheet is created at the portion to be illuminated.

Growing community of inventors

Stanford, CA, United States of America

Ryosuke Itoh