Kikkoman Corporation

Ryoichi Sakaue

Naoki Kajiyama

Yasuji Koyama

Ayumi Arai

A novel cholesterol oxidase having stability in the presence of surfactant and a gene encoding the novel cholesterol oxidase.

Cholesterol oxidase stable in the presence of surfactant

The present invention provides a novel fructosyl amino acid oxidase that is excellent in thermal stability. Moreover, the present invention provides a fructosyl amino acid oxidase gene encoding the novel fructosyl amino acid oxidase, a recombinant DNA in which the gene is inserted into a vector DNA, a transformant or transductant containing this gene, and a process for producing the novel fructosyl amino acid oxidase, which comprises culturing the transformant or transductant in a medium and collecting the novel fructosyl amino acid oxidase from the culture product.

Fructosyl amino acid oxidase

Based on a principle that is different to that of a conventional enzymatic method, the present invention provides a novel method for assaying a glycated protein by a simple procedure, within a short period of time, and with high accuracy, and a reagent kit for assaying used in the method. The method for assaying a glycated protein in a sample is realized by treating a glycated protein-containing sample with protease to liberate a glycated peptide, preferably an &#x3b1;-glycated peptide, particularly preferably an &#x3b1;-glycated dipeptide, from a glycated protein, allowing an oxidase to react with the liberated glycated peptide, and determining the produced hydrogen peroxide.

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Chiba, Japan

Ryoichi Sakaue