Kurashiki Boseki Kabushiki Kaisha

Ryohei Yamamoto

Toyokazu Nishino

Kunihiro Ohshima

Susumu Katsuen

Chikaaki Sakai

Masaru Yamada

Takashi Kondo

Masaharu Nojima

Satoshi Narita

Seiko Kawamura

Hirokuni Arakawa

Hisashi Torishima

Animal adhesive cells, particularly human vascular endothelial cells, are cultured in serum-free condition by coating at least one polymer having cell adhesive activity on an inner surface of a culture vessel or surface of a carrier for cell culture, and culturing the cells in the coated vessel or with the coated carrier using a serum-free medium for animal cell culture containing isolated serum albumin, and preferably also transferrin. The polymer is a synthetic polymer modified with a peptide having cell adhesive activity or a natural polymer having cell adhesive activity or a combination thereof. Preferably, the peptide is RGDV, RGDS, RGDN, DGEA or YIGSR and the natural polymer is collagen, gelatin, keratin, fibronectin, vitronectin or laminin. A preferred medium for culturing human vascular endothelial cells is basal medium MCDB 131 or MCDB 107 containing isolated serum albumin, transferrin, hydrocortisone and epithelial growth factor.

Method for serum-free culture of human vascular endothelial cells

Disclosed is a coating compositions for culturing animal adhesive cells comprising a water-insoluble polymer dissolved in a lower alcohol or an aqueous lower alcohol which enable to enhance the adhesive ability and growth of the adhesive cells. Also disclosed is serum-free cell culturing method using culture vessels or carrier coated with the water-insoluble polymer having cell adhesive activity on at least a part of the surface which enable to not only culture but also subculture a variety of adhesive cells including vascular endothelial cell under serum-free condition.

Coating composition for culturing animal cells and method for culturing

The present invention provides a serum-free medium for culturing animal cells characterized in that it comprises 8.0-14.0 mg/l of methionine. Although a wide variety of animal cells can effectively be cultured by means of the serum-free medium according to the present invention, said serum-free medium produces an excellent growth effect for culturing animal epithelial cells in particular.

Serum-free medium for culturing animal cells

The present invention provides the process for treating the animal hairs by solubilization wherein the animal hairs can be treated in a short time without any complicated operations and special apparatuses, and the process for recovering the solubilized product of the animal hairs wherein said product can be recovered simply and efficiently from the solution thereof. The former is the process for treating animal hairs by solubilization which comprises solubilizing the animal hairs in a weak alkaline liquid medium in the presence of an oxidizing agent whose concentration is high. The latter is the process for recovering a solubilized product of animal hairs which comprises admixing a solution of said product with an organic acid or an aqueous solution thereof to precipitate said product.

Process for solubilizing animal hair

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Takatsuki, Japan

Ryohei Yamamoto