Lumen Bioscience, Inc.

Fred Hutchinson Cancer Center

Seattle Children's Hospital

Lumen Biosciences, Inc.

Fred Hutchinson Cancer Research Center

Ryo Takeuchi

James M Roberts

Barry L Stoddard

Andrew M Scharenberg

David J Rawlings

Mark T Groudine

Karen Sommer

Yuchi Chiang Honaker

Michael A Bender

Nhi Khuong

Rolf Kuestner

Samuel West

Provided herein are novel thermostable phycobiliproteins. These proteins may be stabilized by the introduction of disulfide bonds which stabilize the protein. Modified cells expressing these thermostable phycobiliproteins and methods of making them are also provided.

Thermostable phycobiliproteins produced from recombinant arthrospira

This disclosure describes techniques for creating stable, targeted mutations in() andhaving stable, targeted mutations.

Targeted mutagenesis in spirulina

The present application relates to, inter alia, compositions including proteins for expression in host cells to render them resistant to rapamycin. The application further relates to methods of using the proteins, cells, and compositions disclosed therein for modulating cell signaling and for selective expansion of cells.

Rapamycin resistant cells

Provided are compositions and methods for the treatment of hemoglobinopathies such as thalassemias and sickle cell disease. Compositions and methods include one or more endonuclease(s) or endonuclease fusion protein(s), including one or more homing endonuclease(s) and/or homing endonuclease fusion protein(s) and/or CRISPR endonuclease(s) and/or CRISPR endonuclease fusion protein(s): (a) to disrupt a Bcl11a coding region; (b) to disrupt a Bcl11a gene regulatory region; (c) to modify an adult human &#x3b2;-globin locus; (d) to disrupt a HbF silencing DNA regulatory element or pathway, such as a Bcl11a-regulated HbF silencing region; (e) to mutate one or more &#x3b3;-globin gene promoter(s) to achieve increased expression of a &#x3b3;-globin gene; (f) to mutate one or more &#x3b4;-globin gene promoter(s) to achieve increased expression of a &#x3b4;-globin gene; and/or (g) to correct one or more &#x3b2;-globin gene mutation(s).

Compositions and methods for the treatment of hemoglobinopathies

Targeted mutagenesis in

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Growing community of inventors

Seattle, WA, United States of America

Ryo Takeuchi