Toyo Seikan Group Holdings, Ltd.

Toyo Seikan Kaisha, Ltd.

Toyo Seikan Group Holdings Co., Ltd.

Ryo Suenaga

Takashi Sato

Takahiko Totani

Kyohei Ota

Yoichi Ishizaki

Masahiro Kuninori

Satoshi Tanaka

Masahito Kogure

Osamu Koseki

Tsutomu Iwasaki

Hisao Iwamoto

Maki Mitsuishi

Yoriko Tokita

Makoto Kobayashi

Toshiki Sakaguchi

Ken Kashiwabara

Taku Hosokai

A cell culturing method using a cell culturing bag () in which a plurality of recess portions are formed, including: a bag body () configured from an upper film () and a lower film () which seal a peripheral portion; and a port () mounted on the bag body (), wherein the cell culturing bag () is placed on a placement surface () of a stand (), and while a culture medium (S) is being injected or discharged from the port (), a pressing member () having a bottom surface () substantially parallel to the placement surface () is used to press the upper film () from above.

Cell culturing method and device

Cell culture bag

A cell culturing method and device that uses a cell culture bag () that includes a bag body () configured from an upper surface film () and a lower surface film () having a sealed periphery, and a port () attached to the bag body (), and a plurality of recesses () being formed in the lower surface film (). The method including: a closing-off step for closing off the plurality of recesses () using the upper surface film () by discharging a culture medium (S) contained in the bag body () through the port (); and a releasing step for releasing, in some or all of the plurality of closed-off recesses (), cells or cell aggregates adhering to the inner surfaces of the recesses ().

A vessel manufacturing method includes placing a bag-shaped, film-based vessel on a placement stage with concave portions formed on the stage, introducing fluid into the vessel placed on the stage, and pressing the vessel which is placed on the stage, by a pressing member while heating at least one of the stage and the pressing member. A cell culture vessel is also provided which includes a first vessel wall as a bottom wall and a second vessel wall. The first vessel wall is formed of a flat film having gas permeability. The second vessel wall is disposed in contact with a peripheral edge portion of the first vessel wall, and has a bulge shape protruding relative to the first vessel wall. The first vessel wall is flat at its section other than a region where the first vessel wall is in contact with at least a charge/discharge port.

Method and apparatus for producing container, cell culture vessel, method for culturing cells, method for producing cell culture vessel, and apparatus for producing cell culture vessel

Provided are a multilayer film having excellent gas permeability and excellent handling properties and hence is suited for forming a cell culture container, and a cell culture container formed by using the same. A multilayer film used for forming a cell culture container, comprising: a base material composed of a polyethylene-based resin having a density of 0.87 g/cmto 0.90 g/cm; and an inner layer composed of a polyethylene-based resin having a density of 0.896 g/cmto 0.93 g/cmand forming a cell culture icy surface. A cell culture container is formed by using this multilayer film.

Culturing cells in a multilayer film container

A cell culture container includes a container main body comprising a gas-permeable plastic film, and an injection/discharge port. A peripheral portion of the container main body is sealed, and the container main body has a bulging shape on a top surface side of the container main body. A plurality of concave portions serving as cell culture parts are provided on a bottom surface of the container main body.

Cell culture vessel, support jig for cell culture vessel and cell culture method

Growing community of inventors

Yokohama, Japan

Ryo Suenaga