Dojindo Laboratories

Ryo Sakamoto

Munetaka Ishiyama

Chiaki Matsumoto

Nobuyuki Ozeki

Hidefumi Iwashita

Masaki Murai

Yuriko Fukuoka

The present invention pertains to: an oxidation color developable compound that is represented by general formula (1), that has excellent solubility in water, and that is less affected by a substance coexisting in a sample; and an oxidation color development reagent using the oxidation color developable compound. In general formula (I), R, R, R, and Reach represent a straight-chain or branched alkyl group having 1-6 carbon atoms, X represents a hydrophilic functional group, and L represents &#x2014;(CH)&#x2014; (j represents an integer of 2-10), &#x2014;(CHCHO)&#x2014; (k represents an integer of 1-10), or &#x2014;(CH)&#x2014;Z&#x2014;(CH)&#x2014; (m and n each independently represent an integer of 1-10, and Z represents &#x2014;N(CH)&#x2014;, &#x2014;CONH&#x2014;, &#x2014;NHCO&#x2014;, &#x2014;COO&#x2014;, &#x2014;OCO&#x2014;, &#x2014;NHCOO&#x2014;, &#x2014;OCONH&#x2014;, &#x2014;NHCONH&#x2014;, and &#x2014;(CHNHCO)&#x2014;).

Oxidation color developable compound and oxidation color development reagent

Disclosed is a pH-responsive fluorescent compound, represented by the general formula, which is a novel pH-responsive fluorescent compound capable of being specifically localized in mitochondria within cells, which exhibits strong fluorescence under weakly acidic pH environments in lysosomes, and which is not readily subject to interference from autofluorescence and background fluorescence due to other fluorescent substances within cells. Also disclosed are a composition for detecting mitophagy using the pH-responsive fluorescent compound, and a method for detecting mitophagy within cells. In the general formula, L represents a linker, X represents a pharmaceutically acceptable anion, and Y represents a reactive group that may react with a functional group on a mitochondrial protein to form a covalent bond.

PH responsive fluorescent compound, composition for detecting mitophagy using same, and method for detecting mitophagy within cells

The purpose of the present invention is to provide a water-soluble tetrazolium compound that will form a water-soluble formazan exhibiting long-wavelength absorption and is stable in aqueous solution for a long period and suitable for the quantitative analysis of dehydrogenases or substrates thereof. Disclosed is a water soluble tetrazolium compound expressed by the following general formula (1): wherein each of Rto Rindependently represents hydrogen atom; nitro group; sulfonate group or alkyl, alkoxy, sulfoalkyl or sulfoalkyloxy groups having 1 to 4 carbon atoms; provided that each of at least two of Rto Rindependently represents sulfonate group; or sulfoalkyl or sulfoalkyloxy groups having 1 to 4 carbon atoms; and M represents an alkali metal or ammonium ion.

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Kumamoto, Japan

Ryo Sakamoto