National Technology & Engineering Solutions of Sandia, LLC

Arizona State University

Ryan Wesley Davis

Eric Monroe

Joseph Carlson

Benjamin Chiau-Pin Wu

Anthe George

Nicholas Richard Myllenbeck

Blake A Simmons

John Michael Gladden

Mary Bao Tran-Gyamfi

Aditya Pandharinath Sarnaik

Weihua Wu

Fang Liu

Joseph S Schoeniger

Benjamin C Wu

Arul Mozhy Varman

Tyler Phillips Eckles

Todd W Lane

Sungwhan Kim

Amit Kumar Jha

Arul Mohzy Varman

Oliver Kilian

Nataly Lyn Beck

Ronald C Pate

Muhammad Faisal

The present invention relates to methods of providing a biomass residuum and compositions thereof. In particular examples, the biomass residuum includes one or more high value amino acids, even after removal of mixed alcohol components. In particular, the methods include implementing pre-treatment conditions and employing fermentation conditions including modified organisms.

Enrichment of amino acids from biomass residuum

A fuel for an internal combustion engine includes a Cto Cdialkoxyalkanoate corresponding to formula (I): wherein the Rgroup is —H or a —CHgroup, the Rand R' groups are alkyl groups independently selected to have 1 to 9 carbon atoms; and the Rgroup is selected to have 1 to 9 carbon atoms. The compounds described herein may be used as neat fuels or mixed fuels (with diesel, biodiesel, jet fuel, marine fuel or other fuel compounds) in autoignition or spark ignition engines, such as diesel engines, gasoline (spark ignition) engines, MCCI, Homogeneous Charge Compression Ignition (HCCI) engines, or more generally in Low-Temperature Gasoline Combustion (LTGC) engines (using gasoline-like fuels), that have the high-efficiency advantages of HCCI but can operate with some level of charge inhomogeneities. Methods of making these compounds are environmentally friendly and can be done in a continuous manner.

Alkyl dialkoxyalkanoates as bioderived, high cetane diesel fuels

A chemical upgrading of two high-yield fermentation products to produce a novel biofuel with properties desirable for use in internal combustion engines produces a Cto Calkoxyalkanoate corresponding to formula (I):

Coupling high yield biochemical intermediates for fuel production

The present disclosure relates to terpene synthases capable of producing terpenoids. In one instance, a transformed organism can include terpene synthases or vectors encoding such synthases. One method of employing such synthases and organisms includes protecting an algal culture, in which the produced terpenoid can act as a biocide.

Crop protection in algae by exogenous terpene expression

Microbial enzymes are used for esterification of biomass-derived substrates for production of industrially valuable esters.was used as an efficient platform for recombinant synthesis of fusel lactates such as the green solvent ethyl lactate.

Production of fusel lactates via biocatalysis

A method of improving a ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) to have a higher protein score is disclosed. The method includes the steps of: making a modified RbcL of the RuBisCo, by, on an RbcL unit of the RuBisCo, either substituting Met for Leu, Phe, Val, or Ile or combinations thereof; substituting Lys for Arg, Thr, or His or combinations thereof; or both of these substitutions. The modified RbcL consequently modifies the RuBisCo and is added to a biomass host where it is stable for homologous recombination. Plastid and nucleus integration was observed. Example RbcL sequences are disclosed with the desirable substitutions. The improved RuBisCo can be used as an improved proteinaceous food source for humans and animals.

Engineering RuBisCo for food safety

A fuel for an internal combustion engine includes a Cto Cdialkoxyalkanoate corresponding to formula (I): 

The present invention relates to methods of upgrading biomass to provide useful chemical intermediates, fuels, amino acids, nutrients, etc. In particular examples, the biomass is a by-product of ethanol production and is mainly used as high-protein feed. Described herein are methods for upgrading such biomass, such as by implementing pre-treatment conditions and by employing fermentation conditions including modified organisms.

Biochemical upgrading of high-protein biomass and grain products

A fuel mixture includes a fuel and an octane overboosting additive. Methods of preparing and using such mixtures are disclosed. In particular, the mixture includes an additive that provides octane boosting that produces peak octane at or before a 40% blend and produces at least a 1 octane boost at 10% volume of additive to fuel mixture.

Compounds that induce octane overboosting

A fuel for an internal combustion engine includes a fatty alkyl ether having a formula corresponding to formula (II): wherein x is 1-8, and y is 0 to 3; and the alkyl is an alkyl group having a number of carbon atoms that is less than the number of carbon atoms in the alkyl chain on the opposite side of the oxygen atom. The fatty alky ether can be used as a neat fuel or blend with biodiesel, diesel, ethanol or other fuels. The fatty alkyl ethers are improved in cetane number and cold flow properties over a biodiesel with fatty acid methyl acid methyl ester compounds. This is particularly valuable for compression ignition engines. A method of combustion in several types of engines is also disclosed.

Fatty acid derived alkyl ether fuels for compression ignition

Systems for algae-based water filtration and algae harvesting are described herein. A flow-way comprises a channel and a divider positioned within the channel that separates the channel into sub-channels. The flow-way further includes an algal growth that grows on a sidewall of the channel and/or the divider. The algal growth can be established in the flow-way by positioning a pre-seeded mesh element within the channel, the pre-seeded mesh element having an algae culture growing thereon prior to positioning the pre-seeded mesh within the channel. A harvesting system comprises a hinged container that includes a straining screen. The hinged container is positioned downstream from a flow-way and receives water from the flow-way. Biomass in the water is strained by and accumulates on the screen. Weight of the accumulated biomass causes the hinged container to tip and deposit the accumulated biomass in a second container.

Algal harvesting and water filtration

The present invention relates to terpene synthases capable of degrading precursors into biofuel compounds, such as terpenoid compounds. In one instance, a transformed organism can include such terpene synthases, as well as vectors encoding such synthases. Methods of employing such synthases and organisms are also described herein.

Terpene synthases for biofuel production and methods thereof

The present invention relates, in part, to fuel mixtures and methods of preparing such mixtures. In particular, the mixture includes an alkenol additive that provides octane boosting.

Growing community of inventors

San Jose, CA, United States of America

Ryan Wesley Davis