Bio-rad Laboratories, Inc.

Tzonev, Svilen, S.

Koehler, Ryan, T.

Ryan T Koehler

Svilen Tzonev

Benjamin J Hindson

Serge Saxonov

Michael Ybarra Lucero

John Frederick Regan

Samantha Cooper

Austin So

Luz Montesclaros

Svilen S Tzonev

System, including methods, apparatus, and compositions, for performing a multiplexed digital assay of at least three targets. In an exemplary method, partitions may be formed each including a portion of a same sample. The sample may include a first target that is a reference sequence, a second target that is a variant sequence from a gene of interest, and a third target that is a normal sequence from the gene of interest. The target may be amplified in the partitions and amplification data may be collected. A level of the second target may be determined. A copy number of the third target may be determined from a level of the first target and a level of the third target.

Multiplexed digital assay for variant and normal forms of a gene of interest

Provided herein are methods, compositions, and kits for detecting alleles using a single probe or a single primer set. Also, provided herein are methods, compositions, and kits for detecting allelic variants using a single probe or a single primer set. Also, provided herein are methods, compositions, and kits for determining a polymerization error rate.

Digital assays for mutation detection

Methods of characterizing a nucleic acid library by digital assay.

Library characterization by digital assay

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West Linn, OR, United States of America

Ryan T Koehler