Inscripta, Inc.

University of Colorado

Biota Technology, Inc.

Cargill Inc.

Danmarks Tekniske Universitet

Opx Biotechnologies, Inc.

Celyntra Therapeutics Sa

Ryan T Gill

Andrew Garst

Kevin D Ness

Phillip Belgrader

Don Masquelier

Jorge Bernate

Tanya Elizabeth Warnecke Lipscomb

Michael D Lynch

Ramsey Ibrahim Zeitoun

J Gregory Caporaso

Dan Knights

Rob Knight

Ajay Kshatriya

John W Ely

Paul Henshaw

Tanya Warnecke

Tanya E W Lipscomb

Joel F Moxley

Juhan Kim

Marcelo Colika Bassalo

Chris Lauber

Dan Theodorescu

Andrea Barghetti

James Prestidge, Jr

Tanya Ew Lipscomb

Line Dahl Poulsen

Dominika Joanna Jedrzejczyk

Kenneth Felsenstein

Tanya Warnecke-Lipscomb

The present invention relates to an engineered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system comprising engineered dual guide nucleic acids (e.g., RNAs) capable of activating a CRISPR-Associated (Cas) nuclease, such as a type V-A Cas nuclease. Also provided are methods of targeting, editing, and/or modifying a nucleic acid using the engineered CRISPR system, and compositions and cells comprising the engineered CRISPR system.

CRISPR systems with engineered dual guide nucleic acids

Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

Nucleic acid-guided nucleases

Provided herein, in some embodiments, are methods and compositions for gene editing using a trackable gene library, for example, a barcoded and gene edited library.

Construction and methods of use of a barcoded and gene edited DNA library

Embodiments disclosed herein include novel nucleic acid-guided nucleases, novel guide nucleic acids, and novel targetable nuclease systems, and methods of use. In some embodiments, engineered non-naturally occurring nucleic acid-guided nucleases, can be used with known guide nucleic acids in a targetable nuclease system. In certain embodiments, targetable nuclease systems can be used to edit targeted genomes of humans and other species. In some embodiments, methods include, but are not limited to, recursive genetic engineering and trackable genetic engineering methods.

Constructs, compositions and methods thereof having improved genome editing efficiency and specificity

Described herein are method for generating a vector for editing a cell. The method comprises ligating into a vector that encodes a portion of a gRNA a cassette comprising at least one editing cassette, a promoter, and a gene encoding another portion of the gRNA. Upon ligation, the portion of the gRNA from the editing cassette and the other portion of the gRNA are ligated and form a functional gRNA.

CRISPR enabled multiplexed genome engineering

Described herein are methods and vectors for rational, multiplexed manipulation of chromosomes within open reading frames (e.g., in protein libraries) or any segment of a chromosome in a cell or population of cells, in which various CRISPR systems are used.

Described herein are molecules for editing a cell. The molecules described herein generally comprise the following covalently-linked components and a nucleic acid encoding a guide RNA (gRNA) sequence targeting a target region in a cell and a region homologous to the target region comprising a change in sequence relative to the target region.

There are provided methods, systems and processes for the utilization of microbial and related genetic information for use in the exploration, determination, production and recovery of natural resources, including energy sources, and the monitoring, control and analysis of processes and activities.

Microbiome based systems, apparatus and methods for the exploration and production of hydrocarbons

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

Automated cell processing methods, modules, instruments, and systems

Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

Methods for generating barcoded combinatorial libraries

Described herein are synthetic oligonucleotides for editing a cell. The oligonucleotides described herein comprise the following covalently-linked components: (i) a nucleic acid encoding a guide RNA (gRNA) sequence targeting a target region in a cell; (ii) a region homologous to the target region comprising a change in sequence relative to the target region; and (iii) a site conferring immunity to nuclease-mediated editing.

Growing community of inventors

Denver, CO, United States of America

Ryan T Gill