Lallemand Hungary Liquidity Management LLC

Stellenbosch University

Danstar Ferment Ag

Universiteit Stellenbosch

Ryan Skinner

Aaron Argyros

Trisha Barrett

Charles F Rice

Erin Wiswall

Kevin S Wenger

Nicky Caiazza

John E McBride

Allan C Froehlich

Anne K Warner

Mark Mellon

Emily Stonehouse

Riaan Den Haan

Shital A Tripathi

Haowen Xu

Chhayal V Gandhi

Kristen M Deleault

John S Bardsley

Frank Agbogbo

Indraneel Shikhare

Alan Belcher

Vineet B Rajgarhia

Jennifer Gosselin

Abigail Foster

Yin-Ying Chiu

Elena E Brevnova

Heidi H Hau

Brooks Henningsen

Janet Fisher

Michelle Oeser

J Kevin Kraus

Johannes Van Eijk

Heidi Lau

Elena Brevnova

The present disclosure concerns recombinant yeast host cells expressing cell-associated heterologous food and/or feed enzymes which are expressed during the propagation phase of the recombinant yeast hosts cells. The recombinant yeast host cells can be used in a subsequent production process to make food and/or feed products, for example, baked products.

Cell-associated heterologous food and/or feed enzymes

The present disclosure relates to recombinant yeast host cells having (i) a first genetic modification for reducing the production of one or more native enzymes that function to produce glycerol or regulating glycerol synthesis and/or allowing the production of an heterologous glucoamylase and (ii) a second genetic modification for reducing the production of one or more native enzymes that function to produce trehalose or regulating trehalose synthesis and/or allowing the expression of an heterologous trehalase. The recombinant yeast host cells can be used to limit the production of (yeast-produced) trehalose (particularly extracellular trehalose) during fermentation and, in some embodiments, can increase the production of a fermentation product (such as, for example, ethanol).

Limiting yeast-produced trehalose in fermentation

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

The present disclosure relates to recombinant yeast strains capable of maintaining their robustness at high temperature as well as recombinant proteins expressed therefrom. The present disclosure also provides methods for using the recombinant yeast strain for making a fermentation product. The present disclosure further process a process for making recombinant yeast strains capable of maintaining their robustness at high temperature.

Yeast strains for the expression and secretion of heterologous proteins at high temperatures

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White River Junction, VT, United States of America

Ryan Skinner