Singular Genomics Systems, Inc.

Ryan Shultzaberger

Daan Witters

Eli N Glezer

Martin Maria Fabani

Allen Lipson

Gudrun Stengel

Sabrina Shore

Christopher Jen-Yue Wei

Bharat Sridhar

Timothy Looney

Andrew King

Provided herein are methods of making, amplifying, and sequencing tagged nucleic acid complements, compositions including interposing oligonucleotide barcodes, and kits useful in obtaining long-range sequence data.

Polynucleotide barcodes for long read sequencing

Provided herein are methods for sequencing both strands of a double stranded nucleic acid fragment that improves fidelity and accuracy of a sequence determination compared to traditional next generation sequencing methods. Compositions and kits for use in the methods are also provided.

Linked paired strand sequencing

Disclosed herein, inter alia, are compositions and methods for amplification of nucleic acid templates, including hybridizing a linear polynucleotide to an immobilized primer on a surface, circularizing the linear polynucleotide to form a circular polynucleotide, and extending the primer with a polymerase.

Nucleic acid circularization and amplification on a surface

Disclosed herein, inter alia, are polynucleotides, supports, kits, and methods of use thereof for amplifying, immobilizing, and sequencing polynucleotides.

Amplification oligonucleotides

Growing community of inventors

San Diego, CA, United States of America

Ryan Shultzaberger