Danisco US Inc.

Dupont US Holding, LLC

E.i. Dupont De Nemours and Company

Ryan L Frisch

Hongxian He

Chris Leeflang

Seung-Pyo Hong

Xiaochun Fan

Marc Anton Bernhard Kolkman

Frank Wouter Koopman

Steven D Doig

Ethel Noland Jackson

Anita Van Kimmenade

Carol Marie Fioresi

Cristina Bongiorni

Stacey Irene Robida Stubbs

Shannon Del Chase

Helen Olivia Masson

Felipe Oseas Bendezu

Compositions and methods are provided for variant Cas systems and elements comprising such systems, including, but not limiting to, Cas endonuclease variants, guide polynucleotide/Cas endonuclease complexes comprising Cas endonuclease variants, as well as guide polynucleotides and guide RNA elements that can interact with Cas endonuclease variants. Compositions and methods are provided for genome modification of a target sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system comprising a Cas9 endonuclease variant to provide an effective system for modifying or altering target sequences within the genome of a cell or organism.

Cas9 variants and methods of use

The present disclosure is generally related to compositions and methods for obtainingcells/strains having increased protein production capabilities. Certain embodiments of the disclosure are related to genetically modifiedcells/strains derived from parentalcells/strains comprising a variant rghR2 gene.

Compositions and methods for increased protein production in

Compositions and methods for increased protein production in bacillus licheniformis

The present disclosure is generally related to novel promoter sequences and methods thereof for enhanced protein production insp. (host) cells. As set forth herein, the novel promoter sequences of the disclosure, when operably linked to a gene or open reading frame encoding a protein of interest, are particularly well suited for use in large scale production of industrially relevant proteins.

Promoter sequences and methods thereof for enhanced protein production incells

The instant disclosure is generally related to novelsp. mutants capable of producing increased amounts of industrially relevant proteins of interest. Certain embodiments of the disclosure are related to modifiedsp. cells comprising an introduced polynucleotide encoding a variant GlcT protein. Other embodiments are related to methods and compositions for producing endogenous and/or heterologous proteins of interest in the modifiedsp. (daughter) cells, whereas certain other embodiments are directed to nucleic acid sequences, particularly polynucleotide open reading frame (ORF) sequences, vectors thereof and DNA expression constructs thereof, encoding variant GlcT proteins of the disclosure.

Mutant and genetically modifiedcells and methods thereof for increased protein production

A composition is disclosed that comprises at least one protein component of an RNA-guided endonuclease (RGEN) and at least one cell-penetrating peptide (CPP), wherein the RGEN protein component and CPP are covalently or non-covalently linked to each other in an RGEN protein-CPP complex. The RGEN protein-CPP complex can traverse (i) a cell membrane, or (ii) a cell wall and cell membrane, of a cell. The RGEN protein component of an RGEN protein-CPP complex in certain embodiments can be associated with a suitable RNA component to provide an RGEN capable of specific DNA targeting. Further disclosed are compositions comprising at least one protein component of a guide polynucleotide/Cas endonuclease complex and at least one CPP, as well as methods of delivering RGEN proteins into microbial cells, as well as methods of targeting DNA with RGENs.

Peptide-mediated delivery of RNA-guided endonuclease into cells

Non-conventional yeasts are disclosed herein comprising at least one RNA-guided endonuclease (RGEN) comprising at least one RNA component that does not have a 5'-cap. This uncapped RNA component comprises a sequence complementary to a target site sequence in a chromosome or episome in the yeast. The RGEN can bind to, and optionally cleave, one or both DNA strands at the target site sequence. An example of an RGEN herein is a complex of a Cas9 protein with a guide RNA. A ribozyme is used in certain embodiments to provide an RNA component lacking a 5&#x2032;-cap. Further disclosed are methods of genetic targeting in non-conventional yeast.

Genetic targeting in non-conventional yeast using an RNA-guided endonuclease

Growing community of inventors

Newark, DE, United States of America

Ryan L Frisch