University of Utah Research Foundation

University of California

Ryan J White

Henry S White

Eric N Ervin

Bo Zhang

Gangli Wang

Hakhyun Nam

Richard B Brown

David Alexander Forsyth

Joel M Harris

Francesco Ricci

Jun Ho Shim

Alexis Vallé- Bélisle

Andrew J Bonham

Joshua R Wayment

Provided are unimolecular oligonucleotide probes for detecting a target in a sample. The probes use target binding-induced structural changes to detect the presence of the target in the sample. Also provided are methods of using the probes to detect a target in a sample.

Nucleotide-based probes and methods for the detection and quantification of macromolecules and other analytes

A method which makes it possible to replace a reference pattern (aka texture) on a video of clothing is described. For example, a video of a T-shirt with the text 'UCLA' could be edited to say 'UC Berkeley' with minimal user intervention. The method is novel because it separates the problem into two parts: erasing the old texture (while properly accounting for lighting) and adding the new texture (properly deformed to appear on the shirt. This decomposition creates realistic images with simple tracking models of the cloth. It is believed that this method may be used for targeted audience sales techniques, with static or dynamic replacement images replacing images on warping or otherwise deforming surfaces, in addition to other perspective deformations. Potentially, real-time image replacements using the techniques taught here could be used for live activities or real-time video feeds.

Automated image replacement using deformation and illumination estimation

A nanopore device includes a membrane having a nanopore extending there through forming a channel from a first side of the membrane to a second side of the membrane. The surface of the channel and first side of the membrane are modified with a hydrophobic coating. A first lipid monolayer is deposited on the first side of the membrane, and a second lipid monolayer is deposited on the second side of the membrane, wherein the hydrophobic coating causes spontaneous generation of a lipid bilayer across the nanopore orifice. Sensing entities, such as a protein ion channel, can be inserted and removed from the bilayer by adjusting transmembrane pressure, and adapter molecules can be electrostatically trapped in the ion channel by applying high transmembrane voltages, while resistance or current flow through the sensing entity can be measured electrically.

Nanopore platforms for ion channel recordings and single molecule detection and analysis

A method of preventing non-specific adsorption of proteins onto a surface can include providing a substrate that has a surface on which surface groups are attached. A solution can be applied to the surface that includes a protective reagent having a terminal functional group exhibiting a dipole moment. A monolayer comprising the protective reagent is assembled on the surface by reacting the protective reagent with the surface groups, thereby creating a protected surface. The protective reagent alone is sufficient to confer to the protected surface an increased resistance to adsorption of proteins.

Surfaces resistant to non-specific protein adsorption and methods of producing the same

Provided are fabrication, characterization and application of a nanodisk electrode, a nanopore electrode and a nanopore membrane. These three nanostructures share common fabrication steps. In one embodiment, the fabrication of a disk electrode involves sealing a sharpened internal signal transduction element ('ISTE') into a substrate, followed by polishing of the substrate until a nanometer-sized disk of the ISTE is exposed. The fabrication of a nanopore electrode is accomplished by etching the nanodisk electrode to create a pore in the substrate, with the remaining ISTE comprising the pore base. Complete removal of the ISTE yields a nanopore membrane, in which a conical shaped pore is embedded in a thin membrane of the substrate.

Nanopore electrode, nanopore membrane, methods of preparation and surface modification, and use thereof

Nanopore based ion-selective electrodes and methods of their manufacture as well as methods for their use are disclosed and described. The nanopore based ion-selective electrode can include a pore being present in a solid material and having a nanosize opening in the solid material, a metal conductor disposed inside the pore opposite the opening in the solid material, a reference electrode material contacting said metal conductor and disposed inside the pore, a conductive composition in contact with the reference electrode and disposed in the pore, and an ion-selective membrane. The ion-selective membrane can be configured to isolate the metal conductor, reference electrode material, and conductive composition together within the pore.

Growing community of inventors

Salt Lake City, UT, United States of America

Ryan J White