University System of Maryland

Ryan Gill

William E Bentley

The present invention provides a method of differential display of prokaryotic messages RNA by RTPCR, the method comprising the steps of: adding a first primer mixture to a first nucleic acid sample including a first mixture of mRNA to form a first primer/first nucleic acid sample mixture; adding the first primer mixture to a second nucleic acid sample including a first mixture of mRNA to form a first primer/second nucleic acid sample mixture; incubating the first primer/first nucleic acid sample mixture to produce a first population of cDNA; incubating the first primer/second nucleic acid sample mixture to produce a second population of cDNA; adding a second primer to the first population of cDNA to form a second primer/first population of cDNA mixture; adding the second primer mixture to the second population of cDNA to form a second primer/second population of cDNA mixture; amplifying the second primer/first population of cDNA mixture to produce a third population of cDNA; amplifying the second primer/second population of cDNA mixture to produce a fourth population of cDNA; identifying the presence or level of mRNA in the third population of cDNA; and identifying the presence or level of mRNA in the fourth population of cDNA.

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Boston, MA, United States of America

Ryan Gill