Dow Agrosciences LLC

Corteva Agriscience LLC

Ryan Gibson

Bruce Allen Nagel

Ruihua Ren

Yanxin Star Gao

Jafar Mammadov

Tyler Mansfield

Liang Ye

Sushmitha Paulraj

Siva Prasad Kumpatla

Steven Arnold Thompson

Steven A Thompson

Siva P Kumpatla

A newly identified protein that is encoded by a polynucleotide sequence associated with cytoplasmic male sterility restorer activity (Rf3) is described. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to restore cytoplasmic male sterility in plants. Further applications of the newly identified polynucleotide sequence associated with cytoplasmic male sterility restorer activity include a mutation (rf3) which results in cytoplasmic male sterility. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to result in cytoplasmic male sterility in plants. Methods for detecting the cytoplasmic male sterility restorer (Rf3) and the cytoplasmic male sterility (rf3) gene sequences are further described.

Maize cytoplasmic male sterility (CMS) S-type restorer gene Rf3

The present disclosure provides a method for selecting a plant comprising a functional restorer gene for maize S-type cytoplasmic male sterility comprising the steps of (a) screening a population of plants for at least one marker nucleic acid, wherein the marker nucleic acid comprises an allele linked to the functional restorer gene for maize S-type cytoplasmic male sterility; (b) detecting the marker nucleic acid; (c) identifying a plant comprising the marker nucleic acid; and (d) selecting the plant comprising the marker nucleic acid, wherein the plant comprising the marker nucleic acid further comprises the functional restorer gene for maize S-type cytoplasmic male sterility. The present disclosure also provides methods for restoring fertility in a progeny of an S-type cytoplasmic male sterile plant and methods for transferring an Rf3 gene into a progeny plant.

Maize cytoplasmic male sterility (CMS) S-type restorer Rf3 gene, molecular markers and their use

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Carmel, IN, United States of America

Ryan Gibson