Illumina Corporation

Moon, John A.

Ryan Christopher Smith

Kevin L Gunderson

John A Moon

M Shane Bowen

Frank J Steemers

Kerri York

Andrew Antony Brown

Wayne N George

Michal Lebl

Hongji Ren

Daniel Bratton

Randall Smith

Xiaodong Zhao

Steven H Modiano

Michel Perbost

Embodiments of the present disclosure include methods and compositions for functionalizing molecules, such as oligonucleotides, with functional groups, including polyhistidine tags useful in affinity methods. Some embodiments include methods for modifying and purifying complex mixtures of molecules by exchange of functional tags.

Functionalization and purification of molecules by reversible group exchange

The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

Polymer coatings

A compound has Formula I: A, B, C, D, W, X, Y, and Z are independently selected from hydrogen, optionally substituted C-Calkyl, optionally substituted C-Calkoxy, aryl, aldehyde, protected aldehyde, CH, N, O, S, null, and bond; Q is selected from aldehyde, protected aldehyde, and null, at least one of A, B, C, D, W, X, Y, Z, or Q is an aldehyde or protected aldehyde; the bonds between each of A-B, B-C, C-D, W-X, X-Y, and Y-Z are selected from single bond, double bond, triple bond, and no bond; L is a linker selected from a C-Calkyl, aralkyl, and aryl, any of which is optionally substituted; one or more methylene unit (CH) of the C-Calkyl is optionally replaced by any combination of oxygen, carbonyl(C&#x2550;O), and NH; and Rand Rare independently selected from &#x2014;NRR, halogen, C-Calkoxy, aralkoxy, alkenyloxy, alkynyloxy, and OCHCHCN; Rand Rare independently a C-C, straight chain or branched alkyl group.

Formylpyrrole-based heterocycles for nucleic acid attachment to supports

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San Diego, CA, United States of America

Ryan Christopher Smith