Dow Agrosciences LLC

Sangamo Biosciences, Inc.

Corteva Agriscience LLC

Sangamo Therapeutics, Inc.

Ryan C Blue

Joseph F Petolino

Steven Lee Evans

David Richard Corbin

Jeffrey C Miller

Fyodor Urnov

Steven Robert Webb

Sarah E Worden

Nicole Arnold

Vipula Shukla

Jon Christopher Mitchell

Qihua C Cai

Robbi J Garrison

Michael G Murray

Jianquan Li

Rebecca Ruth Miles

Lisa W Baker

W Michael Ainley

Steven L Evans

Lakshmi Sastry-Dent

Zehui Cao

William M Ainley

Nicole L Arnold

Joseph Petolino

Jon C Mitchell

Jeffrey Miller

William Michael Ainley

Vipula K Shukla

This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.

Plant transactivation interaction motifs and uses thereof

A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

Engineered landing pads for gene targeting in plants

Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.

Optimized non-canonical zinc finger proteins

A universal donor polynucleotide is described that can be inserted at targeted locations in plant genomes to facilitate rapid and high throughput integration of a donor molecule within a specific genomic location.

Universal donor system for gene targeting

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Fishers, IN, United States of America

Ryan C Blue