The Charles Stark Draper Laboratory, Inc.

Ryan A Dubay

Jason Oliver Fiering

Charles A Lissandrello

Rebecca Christianson

Gregg E Favalora

Steven J Byrnes

Vishal Tandon

Kenneth T Kotz

Andrew P Magyar

Ian Ward Frank

Kirsty A McFarland

Melissa M Sprachman

Peter Cavanagh

Jenna Leigh Balestrini

Erin Rosenberger

Stacey Markovic

Haiyao Huang

Emma Vargo

Peter Hsi

Jason Durant

A parallelized chain-synthesizing technique includes capillary tubes, where each tube provides multiple locations or addresses where a specific arbitrary sequence for polymeric chains can be synthesized. An optical addressing system selectively delivers light to the locations to mediate or control reactions in the tubes.

Apparatus for high density information storage in molecular chains

A microfluidic system can include a substrate comprising an elastic material and defining a microfluidic channel. The substrate can have a first set of dimensions defining a thickness of a wall of the microfluidic channel and a second set of dimensions defining a width of the microfluidic channel. A transducer can be mechanically coupled with the substrate. The transducer can be operated at a predetermined frequency different from a primary thickness resonant frequency of the transducer. A thickness and a width of the transducer can be selected based on the first set of dimensions defining the thickness of the wall of the microfluidic channel and the second set of dimensions defining the width of the microfluidic channel.

Microfluidic acoustic separation devices

Transfer of genetic and other materials to cells is conducted in a hands-free, automated and continuous process that includes flowing the cells between electroporation electrodes to facilitate delivery of a payload into the cells, while acoustophoretically focusing the cells. Also described is a control method for the acoustophoretic focusing of cells that includes detecting locations of cells flowing through a channel, such as with an image analytics system, and modulating a drive signal to an acoustic transducer to change the locations of the cells flowing in the channel. Finally, an electroporation driver module is described that uses a digital to analog converter for generating an electroporation waveform and an amplifier for amplifying the electroporation waveform for application to electroporation electrodes.

Method and apparatus for electroporation of acoustically-aligned cells

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Cambridge, MA, United States of America

Ryan A Dubay