Difco Laboratories Incorporated

Other

Biolumix Inc.

Neogen Corporation

Centrus International, Inc.

Ruth F Eden

Gideon Eden

Jerry W Smith

Steven H Boyd

Leon F Strenkoski

Ray M McMillian

Nadine M Sullivan

Amy T Meszaros

Thomas E Thompson

Charles A Abbas

A new device and method for detecting the presence of living microorganisms in test samples are described. The device includes a container having at least one section transparent to light with an incubation zone defined in the container, the incubation zone containing growth media in which the sample is cultured. A detection zone containing a matrix composed of a polymeric material which is substantially transparent to light, and at least one indicator reagent sensitive to carbon dioxide gas generated by the microorganisms in the incubation zone is located in the transparent section of the matrix. The matrix is configured to facilitate penetration of external light aimed at the transparent section of the container and interaction of the external light with the indicator reagent to yield interactive light that escapes through the transparent section of the container, said interactive light is being indicative of the presence and/or concentration of the microorganisms.

[object Object]

A new device and method for detecting the presence of living microorganisms in test samples are described. The device comprises a container with at least one section transparent to light, a growth zone located in said container containing a mixture of growth media capable of supporting growth of the microorganisms, and at least one indicator substrate that changes its optical properties due to growth of the microorganisms. A detection zone is located in the container adjacent to the transparent section, and a barrier layer comprising porous solid material separates the two zones, allowing diffusion of molecules and ions of metabolic by-products of the organisms, while preventing microorganisms and particulate matter of the test sample from penetrating into the detection zone.

Optical method and device for the detection and enumeration of microorganisms

A device and method simultaneously detects and enumerates two groups of microorganisms in a test sample, utilizing a single test container. In the container liquid growth media, a chromogenic substrate and a fluorogenic substrate are mixed with the test sample. The test container is incubated to allow bacterial growth and metabolism. Spectral changes of the substrates are dynamically detected using two external light sources aimed at a transparent section of the test container, and a single external photo detector. One light source operates in the visible band and the second in the long ultraviolet band. The two dynamic time patterns generated by the two substrates are analyzed in real time to determine the presence or absence of each microorganisms group and to enumerate their original concentrations in the test sample.

Device and method for the detection and enumeration of multiple groups of microorganisms

Four-legged vial

A method and device are described to concentrate target organisms from a mixture of organisms. Beads () made of material such as nylon, polystyrene or glass are coated with antibodies of specific microorganisms. The beads () are contained in an enclosure () surrounded by grid material (). The pore size of the grid is smaller than the size of the beads, to assure that the beads stay within the grid material and larger than the size of the microorganisms to allow the interaction of the microorganisms with the beads. A rod () is attached to the upper part of the enclosure () allowing the agitation of the device inside he growth medium containing the target organisms.

Method and device for concentrating selected groups of microorganisms

A culture package system comprising a package preferably in the form of a container containing one or more conventional culture media dishes containing solidified culture media. Each dish includes a bottom wall and a peripheral side wall and a removable cover having a bottom wall and a peripheral side side wall which is loosely telescoped over the dish. A plurality of the dishes are preferably provided in inverted stacked relation in the container. A cover is hermetically sealed to close the container. The interior of the package has a gaseous atmosphere having less than 1% oxygen. An oxygen absorber is placed in the package to facilitate maintaining the atmosphere at less than 1% oxygen. Preferably a moisture absorber is also placed in the package. The package is made of plastic material which is impermeable to oxygen and moisture and preferably is in the form of a container which has a base wall and a peripheral wall and a cover sealed thereto.

Culture dish package and method of making

A device for detecting and enumerating microorganisms in a sample substance employs an external container and an internal container inserted into the headspace of the external container. The sample is mixed with liquid growth media in the internal container. Gases generated from the metabolic activity of the microorganisms during incubation expand to the headspace of the sealed external container and interact with an indicator located at a transparent section of the external container. Changes of the indicator are read by an external real time photometer that generates time patterns for analysis. These patterns indicate the presence or absence of microorganisms and assess the level of contamination in the original sample.

Double container device and method for detecting and enumerating microorganisms in a sample

A system (100), methods and apparatuses for transferring a primary medium (7) after incubating of the medium (7) are described. The system (100) includes a container (1) for containing a growth medium (7) and a sample (8) to be tested for the presence of target microorganisms. A system (100) for transferring a primary medium (7) after incubating of the medium (7) is also described. The system (100) includes an automated pouring mechanism for automatically pouring the primary medium (7) into a plurality of media receiving vessels (11) and a metering mechanism for metering the amount of medium (7) poured into each media receiving vessel (11). An automated tilting apparatus (20) tilts the container (1) to actuate pouring of a predetermined amount of the medium (7) from the container (1) into the media receiver (11). An apparatus (20) for automatically transferring medium (7) for collection and testing of microorganisms is also described. It includes an automated tilting apparatus (20) for automatically tilting the container (1) to actuate pouring of a predetermined amount of medium (7) from the container (1) into a media receiver (11). A container (1) for transferring medium (7) for collection and testing of microorganisms is also described. A manifold (12) for controlling the flow of a primary medium (7) transferred from a from a container (1) to a receptacle vessel (11) is also described.

System and apparatus for automatically transferring media

A container (10) adapted for use in the detection of aerobic microorganisms in a sample includes a non-toxic insert (22) disposed within the container (10) for supporting microorganisms adhered thereto and for increasing microbial exposure to oxygenated growth media to enhance microbial metabolism. A method for making the container (10) includes the steps of inserting a non-toxic insert (22) into the container (10) and adding growth media (14). Also, a method of detecting aerobic microbiological growth in a sealed sample container (10) having a headspace (16) and which contains a sample which may contain an unknown microorganism includes the steps of providing a sealed sample container (10) having a headspace (16) and non-toxic insert (22) saturated with microbiological growth media (24), inoculating the insert (22) within the sealed sample container (10), and monitoring metabolism in the container (10) as an indicator of the presence of microorganisms to detect microorganisms in the sample.

Microbiological culture bottle, and method of making and using same

A test slide (10) for performing diagnostic tests for detecting the presence of cells, their enzymes, metabolites and other cellular derivatives. The test slide (10) comprises a support strip (14), a coating (34) comprising a carrier and a diagnostic reagent disposed on the support strip (14), and a mount (12) comprising a front wall (18) and a rear wall (20). The coating (34) has the properties of being dry and stable, and water soluble. Further, the coating (34) and the diagnostic reagent are capable of rehydration. The support strip (14) is disposed between the front wall (18) and the rear wall (20) of the mount (12). Both the front (18) and rear (20) walls can have coinciding openings (28,28a) that overlie the support strip (14) thereby allowing spectroscopic, visual and microscopic examination of the support strip (14).

Dry slide for diagnostic tests

A new device and method to detect the presence of live microorganisms are described. The device employs a liquid phase which supports microbial growth by utilizing appropriate media. A secondary phase which consists of semi-liquid material forms a barrier layer through which only small particles can diffuse and which prevent the presence of any large particles. Noise-free optical measurements are carried out at the barrier layer which are indicative of metabolic changes associated with microbial growth.

Device and method for use in detecting microorganisms in a sample

A method of detecting, identifying, and enumerating microbes in biological and non-biological samples includes the steps of combining the sample with a triggerable chemiluminescent compound specifically susceptible to the initiation of chemiluminescent decomposition by at least one microbial enzyme in the sample and detecting and integrating light emission over an extended period of time as an indication of the presence, identification, or enumeration of the microbes in the sample.

Method of detecting microbes utilizing chemiluminescent compound

A process and kit for detecting microbial metabolism is disclosed, the process including the steps of combining medium, a metabolic dye having an absorbance spectrum which in the presence of microorganisms changes and responds to metabolism of the microorganism and a second analytical dye having an excitation or emission spectrum which overlaps with one of the changed or unchanged absorbance spectra of the first dye. The medium contains a sample solution to be analyzed for containing metabolizing microorganisms. The user observes either no change in the fluorescence emissions of the second analytical dye indicating the absence of metabolizing microorganisms in the sample or a change indicating the presence of metabolizing microorganisms in the sample.

Growing community of inventors

Ann Arbor, MI, United States of America

Ruth F Eden