The University of Chicago

California Institute of Technology

Harvard College

Slipchip Corporation

Talis Biomedical Corporation

Rustem F Ismagilov

Joshua David Tice

Cory John Gerdts

Bo Zheng

Feng Shen

Lewis Spencer Roach

Jason E Kreutz

Wenbin Du

Bing Sun

Erik B Jue

Helen Song Baca

George M Whitesides

Daan Witters

Paul J A Kenis

Matthew S Curtis

David A Selck

Helen Song

Eugenia Khorosheva

Liang Li

Nathan G Schoepp

Julia A Kornfield

Tao Deng

Delai Chen

Hyun Jung Kim

Katherine T Faber

Stefano Begolo

Travis S Schlappi

James Q Boedicker

Jacob T Barlow

Said R Bogatyrev

Francisco Arias

Noriaki Arai

Espir Kahatt

Mitchell Guttman

Philip Homewood

Hedia Maamar

Yu-Hsiang Hsu

Orland Bateman

Eric J Liaw

Emily S Savela

Kevin Paul Flood Nichols

Mikhail Karymov

Dmitriy V Zhukov

Stephanie McCalla

Weishan Liu

Jesus Rodriguez Manzano

Chris Da Costa

Rosaria Ferrigno

Xingyu Jiang

Philip James Homewood

Qichao Pan

Alexander Winnett

Matthew M Cooper

Joe Baker

Shencheng Ge

Mary Arrastia

David Rosmarin

Justin Rolando

Toan Huynh

Jessamine Lee, Jr

Jason Eugene Kreutz

Provided herein is an antibiotic susceptibility test and related compositions, methods and systems based on detection of a nucleic acid from a target microorganism in a sample in the presence or absence of a lysis treatment of the sample.

Antibiotic susceptibility of microorganisms and related methods and systems

Provided herein are methods and systems for absolute quantification of a target 16S rRNA and/or of a target prokaryotic taxon, based on amplifying and sequencing a same 16S rRNA recognition segment in which target 16S rRNA conserved regions flank 16S rRNA variable regions, conserved and variable among a plurality of sample 16S rRNAs and/or of a sample prokaryotic taxon of higher taxonomic rank with respect to the target taxon. In the methods and systems, absolute abundance of the a plurality of sample 16S rRNAs and/or of the sample prokaryotic taxon detected by the amplifying, is multiplied by the relative abundance of the target 16S rRNA and/or of a target prokaryotic taxon detected by the sequencing to provide the absolute quantification in accordance with method and systems of the disclosure.

Absolute quantification of nucleic acids and related methods and systems

Provided herein are methods and systems and related composition, to provide a rodent model having a target microbiome profile formed by a target presence, a target proportion and/or a target total load of a target prokaryote of a target taxon, based on absolute quantification of the target prokaryote. Further provided are rodents obtained by the methods herein described and related use in testing methods performed in connection with physiological or pathological conditions in an individual preferably a human individual. Also provided herein is a tailcup device and related use to prevent coprophagia in rodents.

Rodent model and related device, compositions, methods and systems

Provided herein are methods for making a freeze-cast material having a internal structure, the methods comprising steps of: determining the internal structure of the material, the internal structure having a plurality of pores, wherein: each of the plurality of pores has directionality; and the step of determining comprises: selecting a temperature gradient and a freezing front velocity to obtain the determined internal structure based on the selected temperature gradient and the selected freezing front velocity; directionally freezing a liquid formulation to form a frozen solid, the step of directionally freezing comprising: controlling the temperature gradient and the freezing front velocity to match the selected temperature gradient and the selected freezing front velocity during directionally freezing; wherein the liquid formulation comprises at least one solvent and at least one dispersed species; and subliming the at least one solvent out of the frozen solid to form the material.

Freeze-cast ceramic membrane for size based filtration

A device for allowing compartmentalized reactions with minimized cross-contamination between the compartments, utilizing a delivery of material by loading wells to pooling wells, such that the pooling wells can be additively provided with reactants while maintaining isolation between the pooling wells. The use of geometric properties is used to facilitate transmission of fluids/droplets without the need for hydrophilic surfaces.

Device for additive delivery of reagents and related methods and systems

The present invention provides microfluidic technology enabling rapid and economical manipulation of reactions on the femtoliter to microliter scale.

Microfluidic system

Methods and systems and related compositions for separating through a solid matrix a mixture comprising a nucleic acid in a concentration of 1 μM or less, together with a target compound having a water solubility equal to or greater than 0.001 g per 100 mL, which can be used for managing fluid flow, biochemical reactions and purification of the nucleic acid or other target analytes. The method comprises contacting the solid matrix with a target compound removing agent having a water solubility equal to or less than 0.05 g per 100 mL at 25° C. at 1 atm pressure with water having a solubility in the removing agent of less than 10 g per 100 mL at 25° C. at 1 atm pressure, the contacting performed to remove the target compound from the solid matrix.

Methods and systems and related compositions for mixtures separation with a solid matrix

Antibiotic susceptibility of microorganisms and related compositions, methods and systems

A sample preparation module accepts a sample including a target analyte. The sample preparation module processes the sample through several reaction chambers and a solid phase column. Different reagents are present in the reaction chambers. The eluted analyte is then transferred to the amplification module, where it is further processed and amplified for optical analysis.

Purification and detection of analytes

Methods and systems and related compositions for separating through a solid matrix a mixture comprising a nucleic acid together with a target compound having a water solubility equal to or greater than 0.01 mg per 100 mL, which can be used for managing fluid flow, biochemical reactions and purification of the nucleic acid or other target analytes.

Provided herein are devices and methods that enable co-incubation of microorganisms. Also provided are methods of making such devices for co-incubation of microorganisms, and various applications of such devices.

Co-incubating confined microbial communities

Methods, systems and related compositions are provided to perform single-cell marking of a nucleic acid and/or protein in a sample based on in-cell or in-organelle barcoding of nucleic acid and/or protein complexes of the cell or organelle; the methods and systems herein described are configured to provide in-cell or in-organelle single-cell marked nucleic acid and/or protein complexes comprising a single-cell, cell-specific, or a single-cell organelle-specific marker.

Methods and systems for performing single cell analysis of molecules and molecular complexes

Disclosed herein are methods and devices for rapid assessment of whether a microorganism present in a sample is susceptible or resistant to a treatment.

Microfluidic measurements of the response of an organism to a drug

Growing community of inventors

Chicago, IL, United States of America

Rustem F Ismagilov