Amprion, Inc.

University of Texas System

Shahnawaz, Mohammad

Jara, Claudio Soto

Vollrath, Benedikt K.

Lebovitz, Russell M.

Russell M Lebovitz

Benedikt K Vollrath

Mohammad Shahnawaz

Luis Concha

Carly Farris

Bret Holguin

Claudio Soto Jara

Frank Espin

Claudio Soto-Jara

Nicolas Mendez Dinamarca

A method is provided for determining the presence of soluble, misfolded α-synuclein protein in a biological sample. The method comprises contacting the biological sample with a pre-incubation mixture, the pre-incubation mixture comprising: a monomeric α-synuclein protein; a buffer composition; a salt; and an indicator, to form an incubation mixture. An incubation cycle is conducted on the incubation mixture in the presence of either a silicon nitride bead or a borosilicate glass bead having a diameter of from about 1 mm to about 5 mm. The method further comprises determining if a detectable amount of misfolded α-synuclein aggregate is present in the biological sample.

Alpha-synuclein detection using beads

Methods and kits are provided for amplifying and detecting misfolded tau protein from samples, for example, from patients having tauopathies such as Alzheimer's Disease, Progressive Supranuclear Palsy, and the like.

Detection of misfolded tau protein

A method is provided for determining the presence of soluble, misfolded &#x3b1;-synuclein protein in a biological sample. The method comprises contacting the biological sample with a pre-incubation mixture, the pre-incubation mixture comprising: a monomeric &#x3b1;-synuclein protein; a buffer composition; a salt; and an indicator, to form an incubation mixture. An incubation cycle is conducted on the incubation mixture in the presence of either a silicon nitride bead or a borosilicate glass bead having a diameter of from about 1 mm to about 5 mm. The method further comprises determining if a detectable amount of misfolded &#x3b1;-synuclein aggregate is present in the biological sample.

Methods and kits are provided for amplifying and detecting &#x3b1;S proteins from samples, for example, from patients having Parkinson's Disease. For example, a method for determining a presence of a soluble, misfolded &#x3b1;S protein may include: contacting the sample with a monomeric, folded &#x3b1;S protein to form an incubation mixture; conducting an incubation cycle two or more times on the incubation mixture effective to form an amplified portion of misfolded &#x3b1;S protein; incubating the incubation mixture effective to cause misfolding and/or aggregation of at least a portion of the monomeric, folded &#x3b1;S protein in the presence of the soluble, misfolded &#x3b1;S protein; physically disrupting the incubation mixture effective to at least partly de-aggregate at least a portion of a misfolded &#x3b1;S aggregate present; and determining the presence of the soluble, misfolded &#x3b1;S protein in the sample by detecting at least a portion of the soluble, misfolded &#x3b1;S protein.

Detection of misfolded alpha synuclein protein

Methods and kits are provided for amplifying and detecting A&#x3b2; proteins from samples, for example, from patients having Alzheimer's Disease. For example, a method for determining a presence of a soluble, misfolded A&#x3b2; protein may include contacting the sample with a monomeric, folded A&#x3b2; protein to form an incubation mixture; conducting an incubation cycle two or more times on the incubation mixture effective to form an amplified portion of misfolded A&#x3b2; protein; incubating the incubation mixture effective to cause misfolding and/or aggregation of at least a portion of the monomeric, folded A&#x3b2; protein; physically disrupting the incubation mixture effective to at least partly de-aggregate at least a portion of a misfolded A&#x3b2; aggregate present; and determining the presence of the soluble, misfolded A&#x3b2; protein in the sample by detecting at least a portion of the amplified portion of misfolded A&#x3b2; protein.

Detection of misfolded amyloid beta protein

Growing community of inventors

Oakland, CA, United States of America

Russell M Lebovitz