Massachusetts Institute of Technology

Boston University

Harvard College

Russell-John Krom

James Joseph Collins

Timothy Kuan-Ta Lu

Robert James Citorik

Michael Andrew Lobritz

Prerna Saluja Bhargava

Provided herein, in some embodiments, are engineered phagemids that comprise at least one synthetic genetic circuit, wherein the at least one synthetic genetic circuit comprises gene sequences encoding at least one non-lytic antimicrobial peptides (AMPs) and/or antibacterial toxin proteins, a stable origin of replication, and a bacteriophage-packaging signal, wherein the engineered phagemid does not comprise some or all gene sequences encoding bacteriophage proteins required for assembly of a bacteriophage particle.

Engineered phagemids

Various aspects and embodiments of the present disclosure are directed to methods and compositions for functionalizing endogenous bacteria in vivo. The methods include delivering to endogenous bacterial cells a recombinant bacteriophage or phagemid that is engineered to contain at least one genetic circuit.

Functionalization of endogenous bacteria

Growing community of inventors

Boston, MA, United States of America

Russell-John Krom