Cp Kelco U.s., Inc.

Pfenex Inc.

Other

Dow Global Technolgoies LLC

Pelican Technology Holdings, Inc.

Russell J Coleman

Yamini N Patel

Nancy E Harding

Diane M Retallack

Charles D Hershberger

Thomas Martin Ramseier

Jane C Schneider

Torben Bruck

Steven Matzke

Charles H Squires

Stacey Lynn Lee

Sol M Resnick

Joseph M Cleary

Thomas M Ramseier

Provided herein are methods of production of recombinantasparaginase. Methods herein produce asparaginase having high expression levels in the periplasm or the cytoplasm of the host cell having activity comparable to commercially available asparaginase preparations.

Method for production of recombinantasparaginase

Provided herein are methods of production of recombinantasparaginase. Methods herein allow production of asparaginase inhost cells at high expression levels and having activity comparable to commercially available asparaginase preparations.

Method for production of recombinant

The present invention provides an array for rapidly identifying a host cell population capable of producing a heterologous recombinant protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous recombinant protein of interest in a periplasm compartment or may secrete the heterologous recombinant protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest including therapeutic proteins, hormones, growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.

Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins

Provided herein are methods of production of recombinantasparaginase. Methods herein allow production of asparaginase in Pseudomonadales host cells at high expression levels and having activity comparable to commercially available asparaginase preparations.

The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene N, M or Istrains containing at least one genetic modification that favors slime-forming polysaccharide production. Methods of making the mutantstrains and the culture broth containing such mutantstrains are also provided.

Targeted gene deletions for polysaccharide slime formers

The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative ofsp. ATCC 53159 that harbors a multicopy broad host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineeredstrain including the needed DNA sequence is encompassed within this invention.

Modified organisms for producing gums

The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene R, M or Nstrains containing at least one genetic modification that favors slime-forming polysaccharide production. Methods of making the mutantstrains and the culture broth containing such mutantstrains are also provided.

The present invention provides an array for rapidly identifying a host cell population capable of producing a heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest including therapeutic proteins, hormones, growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.

The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene R, M or Nstrains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutantstrains and the culture broth containing such mutantstrains are also provided.

The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative ofsp. ATCC 53159 that harbors a multicopy broad host range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineeredstrain including the needed DNA sequence is encompassed within this invention.

Method of producing a diutan gum

The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene Istrains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutantstrains and the culture broth containing such mutantstrains are also provided.

The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene Rstrains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutantstrains and the culture broth containing such mutantstrains are also provided.

The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene M or gene Nstrains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutantstrains and the culture broth containing such mutantstrains are also provided.

Growing community of inventors

San Diego, CA, United States of America

Russell J Coleman