Schering Corporation

Russell Gg Condon

Yair Alroy

Andreas Frei

Vijay Yabannavar

Serge Batandolo

Nancy V Connelly

Edward Glowacki

Jingdong Zhu

This invention relates to methods for the cultivating cells, and in particular to methods for propagating viruses.

Methods for cultivating cells and propagating viruses

An insoluble mammalian protein is extracted from transformed bacteria expressing the mammalian protein while avoiding irreversible insolubilization of bacterial host proteins by homogenizing the fermentation broth, centrifuging the homogenized broth and removing the supernatant liquid for the inclusion body containing pellet. In another embodiment, the pH of the homogenized broth is adjusted to 2.0 prior to centrifugation. The acidified broth is then centrifuged, and the pellet is resuspended in buffer, homogenized again, and the inclusion body is isolated by centrifugation.

Extraction of heterologous insoluble proteins from bacteria

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New Brunswick, NJ, United States of America

Russell Gg Condon