Florida Atlantic University

Other

University of California

Russell G Kerr

Robert S Jacobs

Amber Kohl

An enzyme having diterpene cyclase activity has been purified fromusing a series of chromatography steps. The purified enzyme has an apparent molecular weight of about 47 kilodaltons and an isoelectric point of about 5.1. The purifed enzyme catalyzed the cyclization of geranyl geranyl diphosphate to elisabethatriene.

Methods and compositions for cyclizing diterpenes

Methods for treating, preventing, or inhibiting diseases and disorders associated with inflammation, cell-proliferation, and pain comprising the administration of a compound having the structural formula wherein Ris a hydrogen, alkyl, aryl, hydroxyalkyl, cycloalkyl, cycloalkenyl, carboxylic acid, alkylamino or amide group having from 2 to 20 carbon atoms, R, R, and Rare each independently hydrogen or an acyl residue having from 1 to 6 carbon atoms, Ris hydrogen, CH, or CHOH, and Ris an organo group such as a hydrocarbon having from 1 to 10 carbon atoms are disclosed. Other seco-pseudopterosins and compounds related to pseudopterosins are disclosed.

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Anti-inflammatory compounds derived from Pseudopterorgorgia elisabethae

An enzyme having diterpene cyclase activity has been purified from using a series of chromatography steps. The purified enzyme has an apparent molecular weight of about 47 kilodaltons and an isoelectric point of about 5.1. The purifed enzyme catalyzed the cyclization of geranyl geranyl diphosphate to elisabethatriene.

Diterpene cyclase and methods of use

A fortified enzyme preparation of the gorgonian Pseudopterogorgia americana or Pseudopterogorgia elisabethae has been developed which efficiently transforms a variety of precursor molecules, e.g., sterols, to their corresponding metabolites, e.g., 9(11)-secosteroid in high yield. NAD, NADP, and glutamate dehydrogenase can be used as additives in this enzymatic conversion. In addition to naturally-occurring metabolites, novel secosteroids have been prepared.

Method for producing marine-derived compounds and novel compounds

A fortified enzyme preparation of the gorgonian Pseudopterogorgia americana has been developed which efficiently transforms a variety of sterols to their 9(11)-secosteroid derivative in high yield. NAD, NADP, and glutamate dehydrogenase are key additives in this enzymatic conversion. In addition to naturally-occurring metabolites, novel secosteroids have been prepared.

Growing community of inventors

Boca Raton, FL, United States of America

Russell G Kerr