Boehringer Mannheim Gmbh

Roche Diagnostics Operations, Inc.

Roche Diagnostics Gmbh

Dako A/s

Other

F. Hoffmann-la Roche Ag

Eucodis Bioscience Gmbh

Rupert Herrmann

Hans-Peter Josel

Dieter Heindl

Christian Klein

Herbert Von Der Eltz

Hans-Georg Batz

Uwe Kobold

Hans-Joachim Guder

Thomas Duelffer

Frank Bergmann

Harvey Buck

Werner Guthlein

Manfred Kuhr

Johann Berger

Martina Junius-Comer

Wolfgang Jenni

Carina Horn

Christoph Seidel

Peter Sluka

Jutta Arden-Jacob

Bernd Vogt

Ursula Klause

Wolfgang Weckerle

Rainer Beckert

Erasmus Huber

Joachim Siedel

Troels Koch

Michael Gerg

Herbert Andres

Juergen Spinke

Josef Maier

Eva Hoess

Wilhelm Tischer

Karl-Heinz Drexhage

Gregor Sagner

Axel Schmidt

Hartmut Merdes

Klaus Muhlegger

Michael Grol

Wolfgang Knoll

Helmut Schlumberger

Leopold Von Proff

Gunter Pappert

Herbert Buschek

Albert Geiger

Josef Ritter

Reinhold Hilpert

Martina Junius

Jürgen Spinke

Florian Binder

Michael Vogeser

Andreas Finke

Heribert Maerz

Fritz Topfmeier

Wolfgang Jona

Jorg Issberner

Rudolf Machat

Gunter Lampert

Rosa Isabel Saez Diaz

Wolfgang Kleeman

Hans-Dieter Jakubke

Frank Bordusa

Thomas Welzel

Helmut Oberpriller

Joachim Hones

Waldemar Adam

Fritz Vogtle

Werner Naser

Karl Heinz Drexhage

Harald Ertl

Thorstein Liebermann

Bruno Frommberger

Federic Donie

Daniel Belik

Bernard Koenig

Francis Mueller

Ernst Selbertinger

Dieter Weiss

Hans-Joachim Holtke

Jorg Frantzeskos

The present disclosure relates to novel bis-maleic anhydrides and to the surprising discovery that bis-maleic anhydride cross-linking agents can be used for preservation/fixation of a cell or tissue sample. Various bis-maleic anhydride cross-linking agent scan be used in methods requiring fixation of a cell or tissue sample. These reagents and methods are especially useful in procedures that require that the fixation agent be removed in order to facilitate analysis with other reagents. The inventive reagents and methods make it easier to reliably assay for various proteins, a nucleic acid and the like using analytical methods such as like immunohistochemistry, fluorescence in situ hybridization, RT-PCR, and the like.

Use of a bis-maleic anhydride cross-linking agent for fixation of a cell or tissue sample

Disclosed are pyridinyl-isoquinoline-dione derivatives, methods of producing these derivatives, conjugates comprising the pyridinyl-isoquinoline dione derivatives and (i) a solid support, or (ii) a biomolecule, methods of producing these conjugates as well as the use of these conjugates as quenchers in fluorescence resonance energy transfer (FRET).

Fluorescence quencher molecules

The present invention relates to a method of measuring a vitamin D metabolite in a sample, the method comprising the steps of (a) treating said sample with a vitamin D metabolite releasing reagent under conditions appropriate to release a vitamin D metabolite from vitamin D-binding protein and not to cause protein precipitation, (b) subjecting the treated sample obtained in step (a) to a chromatographic separation, and (c) measuring a vitamin D metabolite during or after said chromatographic separation. The present invention also relates to methods for determining the vitamin D status of a subject, for use in the diagnosis of disease, and to agents and kits for use in performing the methods of the invention.

Measurement of vitamin D

The invention relates to a method for differentially hemolyzing whole blood. It discloses methods for detecting an analyte in a liquid sample known or suspected to contain red blood cells and suspected or known to contain eukaryotic cells, the method including the steps of processing the liquid sample with a membrane solubilizing agent under conditions appropriate to lyse cell membranes of red blood cells and at the same time not to cause precipitation of sample constituents, subjecting the processed sample to a chromatographic separation, and detecting the analyte. The differential hemolysis of red blood cells is of advantage in a method of detecting an analyte in a liquid sample that may contain both erythrocytes and nucleated cells. The differential solubilization of red blood cells can be easily combined with an online detection methodology, like LC-MS, and is advantageous in the detection of many analytes, e.g. in the detection of folate or of immunosuppressive drugs, like tacrolimus or sirolimus.

Differential hemolysis of a whole blood sample

The present invention relates to a sampling tube for collecting and processing a whole blood sample. The sampling tube contains a reagent for differential hemolysis of whole blood, wherein said reagent for differential hemolysis comprises a chemical for differential hemolysis and an anti-coagulant, and wherein said sampling tube is a ready-to-use and single-use sampling tube. It also relates to the use of said sampling tube in the processing of a whole blood sample for liquid chromatography and also to the use of a blood sample processed in such sampling tube in a liquid chromatography-based analysis.

Ready-to-use whole blood collection vessel

The present invention provides a novel procedure of preparing samples for analysis by way of mass spectrometry, preferably LC-MS/MS. Accordingly, functionalized magnetic particles with a hydrophobic surface were used for extracting low molecular weight compounds from complex liquid biological samples such as plasma, serum, whole blood or hemolyzed blood. The method of the invention includes (a) contacting the sample with an amount of functionalized magnetic particles with a hydrophobic surface, (b) incubating the sample and the particles, thereby adsorbing the compound to the hydrophobic surface, (c) separating the particles by applying a magnetic field and removing the liquid, (d) optionally washing the particles, (e) eluting the compound from the particles.

Preparation of samples for LC-MS/MS using magnetic particles

The present invention relates to a reagent for digestion of hemoglobin comprising a buffer, pepsin and a-dialkyl-imidazolium salt. It also discloses to the use of this reagent in a method for digesting hemoglobin, in a method for detecting HbA1c, and to a sampling tube for collection of a whole blood sample comprising said reagent for digesting hemoglobin.

Reagent for digestion of hemoglobin

A method and reagent for detecting the presence or amount of an analyte by a redox reaction and a fluorimetric determination, is disclosed. The reagent comprises a compound of the general formula Q-F as a redox indicator, wherein Q is a quencher group and F is a fluorophore group. The quencher group Q or/and the fluorophore group F can be reduced or oxidized, and the fluorescence can change depending on the reduction or oxidation.

Reagent for fluorimetric determination of analytes

The present invention relates to novel chemiluminescent compounds, to a method for synthesizing these compounds, to derivatives and conjugates comprising these compounds, to the use of these compounds or conjugates thereof in chemiluminescence based assays, especially in immunoassays.

Chemiluminescent compounds and their use

A method and reagent for detecting an analyte by a redox reaction and a fluorimetric determination, is disclosed. The method comprises contacting a sample containing the analyte with a detection reagent which contains a compound of the general formula Q-F as a fluorimetric redox indicator, wherein Q is a quencher group and F is a fluorophore group.

Fluorimetric determination of analytes by an intramolecular quencher-fluorophore conjugate

The present invention concerns a method for producing microparticles loaded with proteins which is characterized in that the microparticles are loaded in suspension under strongly alkaline conditions. The invention also concerns microparticles which can be produced using this method and their use in a binding test e.g. in an immunoassay.

Method for producing microparticles loaded with proteins

The present invention relates to a chemical compound comprising a light emitting moiety precursor and a precursor of a leaving group, bound to each other by an amide or by an ester bond and characterized in that the leaving group precursor upon oxidation is converted into the leaving group. The invention also relates to compounds additionally comprising a coupling group to the use of such compounds for labeling of biomolecules and more generally to the use of such compounds in chemiluminescence detection procedures.

Compounds for chemiluminescense procedures

The present invention concerns new fluorescent dye systems especially for fluorescence resonance energy transfer determinations for example combined with the time-resolved measurement of the resulting fluorescence. The invention also concerns the use of these dyes to label biomolecules and for the homogeneous determination of interactions between biomolecules, for example for detecting an analyte.

Dye pair for fluorescence resonance energy transfer (FRET) measurements

Growing community of inventors

Weilheim, Germany

Rupert Herrmann