Mgi Tech Co., Ltd.

Ruoying Chen

Hui Fu Jiang

Wenwei Zhang

Chunyu Geng

Lingyu He

Rongrong Guo

Radoje Drmanac

Yuan Jiang

Xia Zhao

Andrei Alexeev

Yingxin Zhang

Yaqiao Li

Hui Jiang

Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.

Method for constructing nucleic acid single-stranded cyclic library and reagents thereof

Provided in the present invention are a method for constructing a nucleic acid single-stranded cyclic library and reagent kit thereof. The method comprises the steps of using a transposase embedding complex to randomly break nucleic acids and connect a first linker; connecting a second linker at a gap; performing a first PCR reaction, wherein the 5' end of one of the primers has a first affinity tag, resulting in a product with two ends connected to different linker sequences; binding the product to a solid vector having a second affinity tag; degenerating and separating single strands having no affinity tag; and cyclizing the single strands.

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Shenzhen, China

Ruoying Chen