Dow Agrosciences LLC

Corteva Agriscience LLC

Ruihua Ren

Bruce Allen Nagel

Siva Prasad Kumpatla

Yanxin Star Gao

Jafar Mammadov

Ryan Gibson

Steven Arnold Thompson

Tyler Mansfield

Sushmitha Paulraj

Liang Ye

Mustafa George McPherson

Peizhong Zheng

Kelly Parliament

Ramesh Buyyarapu

Chandra Channabasavaradhya

Joseph W Spinks

Van Leonard Ripley

Robert Martin Benson

Thomas W Greene

Angela Lee Erickson

David Herbert Meyer

Wenxiang Gao

Steven A Thompson

Chandra-Shekar A Channabasavaradhya

Gary L Cutter

Leonardo Velasco

Begona Perez Vich

Jose Maria Fernandez Martinez

Milan Avery

Siva P Kumpatla

A newly identified protein that is encoded by a polynucleotide sequence associated with cytoplasmic male sterility restorer activity (Rf3) is described. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to restore cytoplasmic male sterility in plants. Further applications of the newly identified polynucleotide sequence associated with cytoplasmic male sterility restorer activity include a mutation (rf3) which results in cytoplasmic male sterility. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to result in cytoplasmic male sterility in plants. Methods for detecting the cytoplasmic male sterility restorer (Rf3) and the cytoplasmic male sterility (rf3) gene sequences are further described.

Maize cytoplasmic male sterility (CMS) S-type restorer gene Rf3

The present disclosure provides a method for selecting a plant comprising a functional restorer gene for maize S-type cytoplasmic male sterility comprising the steps of (a) screening a population of plants for at least one marker nucleic acid, wherein the marker nucleic acid comprises an allele linked to the functional restorer gene for maize S-type cytoplasmic male sterility; (b) detecting the marker nucleic acid; (c) identifying a plant comprising the marker nucleic acid; and (d) selecting the plant comprising the marker nucleic acid, wherein the plant comprising the marker nucleic acid further comprises the functional restorer gene for maize S-type cytoplasmic male sterility. The present disclosure also provides methods for restoring fertility in a progeny of an S-type cytoplasmic male sterile plant and methods for transferring an Rf3 gene into a progeny plant.

Maize cytoplasmic male sterility (CMS) S-type restorer Rf3 gene, molecular markers and their use

This disclosure concerns methods and compositions for identifying cotton plants that have a reniform nematode resistance trait. Some embodiments concern molecular markers to identify, select, and/or construct reniform nematode resistant plants and germplasm, or to identify and counter-select relatively susceptible plants. This disclosure also concerns cotton plants comprising a reniform nematode resistance trait that are generated by methods utilizing at least one marker described herein.

Markers linked to reniform nematode resistance

This invention relates to methods for identifying sunflower plants that having increasedresistance. The methods use molecular markers to identify and to select plants with increasedresistance. Maize plants generated by the methods of the invention are also a feature of the invention.

Molecular markers associated withresistance in sunflower

A method is provided for determining the zygosity of an Rf4 gene in a corn plant. A method may include performing a first PCR assay, a second PCR assay, quantifying probes used in the first and second PCR assays, and comparing the quantified probes to determine zygosity.

Endpoint zygosity assay to detect Rf4 gene in maize

This disclosure concerns high-resolution mapping and candidate gene cloning of Rf4, a maize restorer of fertility gene that restores fertility to C-type cytoplasmic male sterility. The disclosure also relates to molecular markers that are tightly-linked to, or reside within, the Rf4 gene. In some embodiments, methods are provided whereby hybrid seeds may be produced from crosses of a male plant comprising nucleic acid molecular markers that are linked to or that reside within the Rf4 gene and a female plant carrying C-type CMS.

Maize cytoplasmic male sterility (CMS) C-type restorer RF4 gene, molecular markers and their use

Growing community of inventors

Carmel, IN, United States of America

Ruihua Ren