Tsinghua University

Zhuhai Shu Tong Medical Technology Co., Ltd.

Rui Tian

Qian Zhou

Hao Dong

Jinchao Zhang

Kai Ni

Jinchao Pang

Mingfei Xu

Hui Zhang

Long Huang

Hongxian Xie

The disclosure relates to a Type II CRISPR/Cas9 genome editing system, belonging to the technical field of genome editing. The genome editing system comprises a Cas9 protein, helper proteins, a CRISPR RNA and a trans-activated CRISPR RNA; wherein the Cas9 protein is a DNA endonuclease, and the Cas9 protein has an amino acid sequence as shown in SEQ ID NO: 1, or an amino acid sequence with at least 80%, 85%, 90%, 95%, 98%, or 99% homology to the amino acid sequence as shown in SEQ ID NO: 1. According to the disclosure, through bioinformatics analysis, the Type II CRSIPR/Cas9 genome editing system in theis discovered, and the genome editing system is applied to editing prokaryotic or eukaryotic genes and provides a new selection for a genome editing toolbox.

Type II CRISPR/Cas9 genome editing system and the application thereof

A design method of a spectrometer and a spectrometer are disclosed, including the following steps: 1) determining an incident angle of a second incident slit and a groove-shaped cycle of a concave grating; 2) estimating a blaze angle of the concave grating, determining a surface material and a groove-shaped structure of the concave grating; 3) acquiring wavelength-diffraction efficiency curves; 4) determining values of incident angles &#x3b8;and &#x3b8;and values of wavelengths &#x3bb;and &#x3bb;, and setting &#x3bb;to equal &#x3bb;; 5) acquiring a record structural parameter and a use structural parameter; 6) determining a manufacture parameter of the concave grating; 7) determining locations of the three incident slits and the three photodetectors relative to the concave grating. The spectrometer acquired by using this method has relatively high diffraction efficiency in most spectrum regions and effectively alleviates the problem of relatively low diffraction efficiency in a broad spectrum region.

Method of spectrometer and spectrometer

The invention provides an exposure method of a holographic grating and an exposure light path. The exposure method includes: (1) determining initial positions (C, D) of the two exposure light sources (S, S); (2) calculating imaging quality parameters of the grating; (3) setting a compensating mirror (A) in the initial light path; (4) adjusting a position of the exposure light source (S) to a new position (D) according to a position of the compensating mirror (A); (5) calculating imaging quality parameters of the grating; (6) judging whether the imaging quality parameters in the step (5) and the imaging quality parameters in the step (2) are equal, and if yes, using the new position (D) as a final position of the exposure light source (S). The exposure method and exposure light path may effectively solve a problem of a much too close distance between exposure light sources.

Exposure method of holographic grating and exposure light path

A manufacture method for a blazed concave grating is provided, including: replicating a blazed concave grating by means of a replication process, after the replication is completed, conducting heat preservation and cooling, then separating two gratings (G, G) by using different pulling manners respectively, and finally splicing the replicated and separated concave grating blanks, to obtain a required blazed concave grating. This manufacture method can solve the problem of a big error in an existing replication method for a blazed concave grating.

Manufacture method for blazed concave grating

Growing community of inventors

Guangdong, China

Rui Tian