Roche Sequencing Solutions, Inc.

Ventana Medical Systems, Inc.

Kapa Biosystems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Rui Chen

Toumy Guettouche

Aaron Richardson

Loida Navarro

Jigar Patel

Jenny A Johnson

Jan Berka

Sedide Ozturk

Nelson Alexander

Stacey Stanislaw

Martin Ranik

Aoune Barhoumi

Seoyoung Kim

Ashley Hayes

Florian Rubelt

Lisa L Gallegos

Severine Margeridon

Alexandra Hui Wang

Aruna Arcot

Maeve E O'Huallachain

Persis Wadia

Daleen Badenhorst

Toummy Guettouche

Nelson R Alexander

Maeve E O'HUALLACHAIN

The invention is a novel method of generating a library of circular single stranded nucleic acid molecules by utilizing circular capture molecules. The method is not limited by size of target nucleic acid molecules and can potentially accommodate very long molecules. The method finds application in nucleic acid sequencing, e.g., nanopore sequencing where unlimited-length templates can be read.

Single stranded circular DNA libraries for circular consensus sequencing

The invention is a novel method of making and using a template for nucleic acid sequencing. The templates include circular and linear templates with symmetric and asymmetric adaptors. The methods include utilizing the templates in an asymmetric fashion.

Determining the sequence of a double-stranded target nucleic acid by employing a terminal transferase, forming a linear joint molecule, and sequencing in one direction

The invention is a novel method of separately sequencing each strand of a nucleic acid involving the use of an adaptor comprising a strand cleavage site or a strand synthesis termination site. The adaptor may also be self-priming at the strand cleavage site.

Generation of single-stranded circular DNA templates for single molecule sequencing

The invention is a method of identifying a cognate antigen for a T-cell receptor using neoantigens from a patient's tumor cells combined with the patient's T-cells and using cell sorting, genome sequencing, expressing TCR genes, presenting tumor neoantigens on MHC complex and uniquely barcoding the T-cells where TCR recognition occurs to tag all components of the TCR recognition complex.

System for identification of antigens recognized by T cell receptors expressed on tumor infiltrating lymphocytes

Asymmetric templates and asymmetric method of nucleic acid sequencing

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Fremont, CA, United States of America

Rui Chen