Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Ruediger Rueger

Anne Stern

Georg Tiefenthaler

Gwen Nichols

Bernhard Goller

Claudio Bordignon

Gong Chen

Christoph Kessler

Karl-Heinz Schleifer

Hua Zhong

Wolfgang Ludwig

Bernhard Reis

Steven D Blotner

Lori Jukofsky

A titer of retroviral vectors of at least about 10 colony-forming units/ml cell culture supernatants is obtained using an amphotropic retroviral packaging cell line which contains functional gag, pol and env genes integrated into the genome in which the expression of the genes gag and pol is regulated independently of the expression of the env genes, wherein the genome of the said cell line contains one or two functionally active env genes.

Amphotropic retrovirus packaging cell line, process for its production and use thereof

For the genus-specific or/and species-specific detection of bacteria in a sample liquid, bacterial RNA is hybridized with a primer which is complementary to a genus-specific or species-specific region of the RNA of particular bacteria or to a highly conserved region of the RNA of bacteria in general, but which is not complementary at its 3&prime; end to the RNA of bacteria of another genus or species, the primer is elongated in the presence of a suitable polymerase and the four deoxyribonucleotides, if desired, with a concurrent or subsequent labelling of the elongation product and an elongation product formed is hybridized with a genus-specific or species-specific oligonucleotide after denaturation and the hybridization is detected by means of the oligonucleotide label.

Method for the genus-specific or/and species-specific detection of bacteria in a sample liquid

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Huglfing, Germany

Ruediger Rueger