Rhodia Operations

Eviagenics, S.a.

Erber Aktiengesellschaft

Rudy Pandjaitan

Odile Ramaen

Mirjana Gelo-Pujic

Jan Modregger

Christina Schwab-Andics

Vincent Sauveplane

Alejandro Luque

Peter Remler

Marc Berlinger

Manuela Herrmann

Henryk Kalisz

A cell may include heterologous polynucleotides encoding a multienzyme complex involved in the metabolic pathway of phenylpropanoids and biosynthesis of a vanilloid or a hydroxybenzaldehyde precursor thereof, which multienzyme complex comprises enzymes for the biosynthesis of coumaric acid and a crotonase.

Recombinant host cell for biosynthetic production

The present invention relates to methods for producing vanilloid compounds in a recombinant host, and in particular for converting a protocatechuic aldehyde into a substantially pure vanilloid. It further relates to novel yeast strains that are suitable for producing such vanilloid compounds.

Selectivity of the production of vanilloids in a recombinant unicellular host

The invention relates to a method for generating a gene mosaic by somatic in vivo recombination, comprising: e) in a single step procedure (vii) transforming a cell with at least one gene A having a sequence homology of less than 99.5% to another gene to be recombined that is an integral part of the cell genome or presented in the framework of a genetic construct, (viii) recombining said genes, (ix) generating a gene mosaic of the genes at an integration site of a target genome, wherein said at least one gene A has a single flanking target sequence either at the 5' end or 3&#x2032; end anchoring to the 5&#x2032; or 3&#x2032; end of said integration site, and f) selecting clones comprising the gene mosaic, as well as a method of producing a diversity of gene mosaics and gene assembly.

Method of generating gene mosaics

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Maisons-Alfort, France

Rudy Pandjaitan