Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Olympus Corporation

Evotec Oai Ag

Rudolf Rigler

Manfred Eigen

Karsten Henco

Wolfgang Simm

Michael Stuke

Ulo Mets

Jerker Widengren

Michael Brinkmeyer

Carl-Roland Rabl

Olaf Lehman

Thomas M Jovin

Olaf Lehmann

Lutz Bodo Veil

Hans Lehrach

Hermann Brundl

Thomas J Jovin

Leo C De Maeyer

Detlev Riesner

A Method for identifying one or a small number of molecules, especially in a dilution of &#x2266;1 &#x3bc;M, using laser excited FCS with measuring times &#x2266;500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably &#x2266;10l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined. The device which can be preferably used for performing the method according to the invention is a per se known system of microscope optics for laser focusing for fluorescence excitation in a small measuring compartment of a very diluted solution and for imaging the emitted light in the subsequent measurement through confocal imaging wherein at least one system of optics with high numerical aperture of preferably &#x2267;1.2 N.A. is employed, the light quantity is limited by a confocally arranged pinhole aperture in the object plane behind the microscope objective, and the measuring compartment is positioned at a distance of between 0 and 1000 &#x3bc;m from the observation objective.

Method and a device for the evaluation of biopolymer fitness

The method according to the invention permits a selected withdrawal of one or a few molecularly disperse or cellular components of a system, such as molecules, molecular complexes, vesicles, micelles, cells, optionally together with an associated volume element V having a size of 10l&#x2267;V&#x2267;10l from a larger sample volume. The selected transfer of the sought component to another environment is effected by defining the space and time of withdrawal by means of a signal correlating with the small component to be withdrawn. The method is particularly useful for the withdrawal of non-abundant components the existence of which can be detected in a preceding step by a scanning process. The method is also useful for the withdrawal of per se unidentified components.

Method and device for the selective withdrawal of components from complex mixtures

A method for identifying one or a small number of molecules, especially in a dilution of &lE;1 &mgr;M, using laser excited FCS with measuring times &lE;500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably &lE;10 l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined.

A method for the direct identification of few, preferably single nucleic acid strands of a specific target sequence (10) in a test solution comprising the following steps:

Method for direct identification of few nucleic acid strands

A reaction kinetics measuring system which provides for the application of great variety of reaction initiating methods, as temperature or pressure or electrical field jump methods, mixing methods, flash photolysis method, and combinations of said methods, and for various optical detection methods, as absorption or fluorescence or scattered light or polarized light detection methods. The system features quickly and easily interchangeable optical elements in a number of optical paths, and a multitude of selectively usable measuring cells to adapt the system to the reaction initiating and detection methods used so that reliable and exactly reproducible results are obtained, which are comparable even if obtained under widely differing conditions.

Apparatus for investigating fast chemical reactions by optical detection

An apparatus for investigating the course of fast chemical reactions, which are initiated in a liquid chemical system under investigation by an external perturbation, e.g. a steep temperature rise (temperature jump). The apparatus comprises first and second light paths conveying a probing light beam and a sense light beam, respectively, and an optical system of extremely high aperture which allows a wide variety of types of measurements, including absorption, fluorescense, fluorescense polarization, and the like with a high signal-to-noise ratio even of small sample concentrations.

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Gottingen, Germany

Rudolf Rigler