Gnothis Holding Ag

Other

Evotec Technologies Gmbh

Gnothis Ab

Gnothis Holding Sa

Rudolf Rigler

Lars Edman

Margo Tiiman

Daniel Rönnlund

The invention relates to a process and a device for analyzing single molecules, particularly to the parallel analysis of a plurality of single molecules. It is suitable for detecting interactions, e.g. binding between single molecules and/or reactions, e.g. elongation or degradation of single molecules. Particularly, the process of the invention relates to the sequencing of single nucleic acid molecules. The single molecule to be analyzed is present in free form, i.e. dissolved or suspended in a liquid medium, within a reaction space formed around the sample spot. According to the present invention, an electrical field is applied across the reaction space, whereby a concentration of single molecules, at the sample spots is effected.

Single molecule analysis in an electrical field

The invention relates to a process for analysing single molecules, in particular for sequencing of single nucleic acid molecules.

Single molecule analysis with high accuracy

An apparatus for characterizing luminescent entities by excitation comprising: &#x2022; a substrate () being in contact with a solution comprising luminescent entities; &#x2022; a source of electromagnetic radiation () providing at least a primary beam of radiation (); an objective (); a first optical element () capable of transforming the intensity profile of the primary beam () into an arbitrary secondary intensity profile (distribution) (); a second optical element () capable of separating (discriminating) radiation by wavelength; and a detector (), where the arbitrary secondary intensity profile has at least an off-center circular continuous intensity distribution () focused on the back focal plane () of the objective forming a collimated beam () capable of creating an evanescent field on the side of the substrate where the solution comprising luminescent entities are located, where the evanescent field excites the luminescent entities thereby creating emission radiation separated by the second optical element () and captioned by the detector (). The invention also relates to an apparatus comprising two optical elements providing a final third intensity profile (distribution) which is the convolution of two mathematical transformations corresponding to each of optical element one and four, respectively.

Apparatus for characterizing luminescent entities

The invention relates to a process and a device for analysing single molecules, particularly to the parallel analysis of a plurality of single molecules. It is suitable for detecting interactions, e.g. binding between single molecules and/or reactions, e.g. elongation or degradation of single molecules. Particularly, the process of the invention relates to the sequencing of single nucleic acid molecules. The single molecule to be analysed is present in free form, i.e. dissolved or suspended in a liquid medium, within a reaction space formed around the sample spot. According to the present invention, an electrical field is applied across the reaction space, whereby a concentration of single molecules, at the sample spots is effected.

The invention relates to a process for parallel high throughput sequencing of nucleic acid molecules, in particular in the single molecule format.

Cyclic single molecule sequencing process

The invention relates to a method for selecting charged particles possessing a predetermined property from a population of a multiplicity of different particles, and also to a device which is suitable for implementing the method.

Selection of particles in laminar flow

In a method for the fluorescence spectroscopic, in particular fluorescence correlation spectroscopic study of a measurement sample, the latter is introduced into a sample holding chamber () formed hollowed in a sample support (). Electrically charged analytes contained in the measurement sample are then concentrated in a measurement volume () lying inside the sample volume, whereupon the measurement volume () is studied. According to the invention, the boundary walls () of the sample holding chamber () are brought essentially surface-wide to an electrostatic potential having the same sign as the charge of the analytes in order to concentrate the analytes in the measurement volume (). An electrical molecule trap formed in this way can be produced very straightforwardly and is suitable in particular for screening studies of measurement samples, a large multiplicity of which are arranged close together in indentations () of the sample support ().

Method for examining a test sample by means of fluorescence spectroscopy, especially fluorescence correlatin spectroscopy, and device for carrying out said method

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Djursholm, Sweden

Rudolf Rigler