Boehringer Mannheim Gmbh

Rudiger Rueger

Christoph Kessler

Gerd Assmann

Harald Funke

Stephan Rust

Rudolf Seibl

Udo Reischl

Process for the production of nucleic acids by hybridization of at least one primer to a template nucleic acid and enzymatic elongation of the primer to a nucleic acid strand which is complementary to the template nucleic acid by reaction with different types of nucleoside triphosphates, wherein the complementary nucleic acid strand carries two or more groups which enable it to be immobilized and one or more detectable groups, a method which uses this process for the detection of nucleic acids, suitable reagent kits therefor and modified nucleic acids.

Process for the production of modified nucleic acids

Method of detecting nucleic acids whose nucleotide sequences differ from one another at a position X. The method uses a set of oligonucleotides where the nucleotides found at position X are complementary to a variant nucleic acids only at this position X. However, with respect to the position of another mismatch Y, the nucleotide sequences thereof differ from one another. The invention further addresses reagents for performing the methods as well as reagents and applications of the method.

Method of detecting variant nucleic acids using extension

Method of detecting variant nucleic acids

Process for the production of ribonucleic acids or for the detection of template nucleic acids based on a transcription technique using two oligonucleotides which can hybridize to adjacent regions on the template nucleic acid without ligation of these oligonucleotides.

Process for the specific production of ribonucleic acids

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Seeshaupt, Germany

Rudiger Rueger