Hoechst Aktiengesellschaft

Rudiger Marquardt

Gerhard Wohner

Susanne Grabley

Klaus Bartsch

Alfred Puhler

Hans-Matthias Deger

Wolfgang Wohlleben

Johann Then

Walter Arnold

Eckhard Strauch

Renate Alijah

Dieter Brauer

Gunter Muth

Martyn K Robinson

Klaus Kuhlein

Gerhard Kretzschmar

Dieter Duwel

Johannes Meiwes

Peter Hammann

Klaus-Peter Koller

Michael Dorschug

Lothar Elling

Maria Regina Kula

Brigitte Horsch

Astrid Zervosen

Evelyn L Doherty

Andrew Doherty

Andreas Seiffert-Storiko

Method for the enzymatic galactosylation of monosaccharides and

L-tertiary-Leucine and L-phosphinothricine are obtainable by transamination of the corresponding keto acids as a precursor in the presence of amino acids as amino group donors. The reaction is preferably carried out with microorganisms or their transaminases.

Process for preparing L-tertiary-leucine and L-phosphinothricine by

Selection of Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistance selectants. The DNA fragment which carries the phosphinothricin(PTC)-resistance gene is obtained from the total DNA of these selectants by cutting with BamHI, cloning of a fragment 4.0 kb in size, and selection for PTT resistance. This gene is suitable for the production of PTC-resistant plants, and as a resistance marker and for the selective N-acetylation of the L-form of racemic PTC.

Phosphinothricin-resistance gene, and its use

Process for preparing L-tertiary-Leucine and L-phosphinothricine by

A process is described to specifically hydrolyze the amino acid chain of preproinsulin analogs to give the corresponding insulins. The hydrolysis is catalyzed by clostripain and, if necessary, carboxypeptidase B.

Clostripain catalyzed hydrolysis of preproinsulin analogs into

The ilvE gene from the strain E. coli ATCC 11303 is suitable for achieving overproduction of the coded aliphatic transaminase and thus for preparing the branched-chain amino acids leucine, isoleucine and valine from the corresponding keto precursors.

Cloning and use of the transaminase gene ilvE

The invention relates to elaiophylin derivatives of the formulae (I), (I'), (III) and (III') ##STR1## in which the C.dbd.C double bonds in the 2,3 and 4,5 position may also be hydrogenated and in which R.sup.1, R.sup.2, R.sup.3, R.sup.4 and X have the meanings indicated. The invention furthermore relates to a process for the preparation of these elaiophylin derivatives, and the use thereof as pharmaceuticals, in particular as anthelmintic pharmaceuticals.

Basic cleavage products of elaiophylin and elaiophylin derivatives and

The isolation of the tyrB gene contained in E. coli ATCC 11303 and its cloning onto a multicopy plasmid results in a 10-fold increase in the L-phenylalanine yield after the transformation of the starting strain with this plasmid.

Cloning and use of transaminase gene tyrB

Intensely crimson colonies are obtained from Streptomyces coelicolor DSM 3030 by total digestion of the total DNA, cloning into a suitable vector, and transformation of Streptomyces lividans. Re-isolation of the plasmid DNA and cutting with BamHI results in fragments about 3.4 to 9 kb in length, on which is located the gene which determines production of the coloring agent. This gene is suitable as marker, in particular as inactivation marker, in Streptomycetes.

Color marker for clonings in Streptomyces lividans

Intensely blue-black colonies are obtained from Streptomyces coelicolor DSM 3030 by total digestion of the total DNA, cloning into a suitable vector, and transformation of a streptomycetes recipient strain. Re-isolation of the plasmid DNA and cutting with BamHI results in a 5.5 kb fragment on which the gene for the coloring agent is located. This gene is suitable as marker, in particular as inactivation marker, in Streptomycetes.

Color marker in streptomycetes plasmids

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Frankfurt am Main, Germany

Rudiger Marquardt