Glycozym Aps

Glycozym USA Inc.

Rudi Steffensen

Henrik Clausen

Eric Bennett

A novel gene defining a novel enzyme UDP-galactose: &#x3b2;-D-galactose-R 4-&#x3b1;-D-galactosyltransferase, termed &#x3b1;4Gal-T1, with unique enzymatic properties is disclosed. The invention provides isolated DNA molecules and DNA constructs encoding &#x3b1;4Gal-T1 and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting &#x3b1;4Gal-T1 activity, as well as cloning and expression vectors including such DNA, host cells comprising DNA encoding &#x3b1;4Gal-T1, and recombinant methods for providing &#x3b1;4Gal-T1. The enzyme &#x3b1;4Gal-T1 and &#x3b1;4Gal-active derivatives thereof are disclosed. Further, the invention discloses methods of obtaining &#x3b1;1, 4galactosyl glycosylated glycosphingolipids by use of an enzymatically active &#x3b1;4Gal-T1 protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active &#x3b1;4Gal-T1 protein as an expression system for recombinant production of such glycosphingolipids. Also a method for the identification of DNA sequence variations in the &#x3b1;4Gal-T1-coding exon by PCR, and detecting the presence of DNA sequence variation, are disclosed.

UDP-galactose:&#x3b2;-D-galactose-R 4-&#x3b1;-D-galactosyltransferase, &#x3b1;4gal-T1

UDP-galactose: &#x3b2;-D-galactose-R 4-&#x3b1;-D-galactosyltransferase, &#x3b1;4Gal-T1

A novel gene defining a novel enzyme UDP-Galactose: &#x3b2;-D-Galactose-R 4-&#x3b1;-D-galactosyltransferase, termed &#x3b1;4Gal-T1, with unique enzymatic properties is disclosed. The invention discloses isolated DNA molecules and DNA constructs encoding &#x3b1;4Gal-T1 and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting &#x3b1;4Gal-T1 activity, as well as cloning and expression vectors including such DNA, cells transfected with vectors, and recombinant methods for providing &#x3b1;4Gal-T1. The enzyme &#x3b1;4Gal-T1 and &#x3b1;4Gal-active derivatives thereof are disclosed. Further, the invention discloses methods of obtaining &#x3b1;1, 4galactosyl glycosylated glycosphingolipids by use of an enzymatically active &#x3b1;4Gal-T1 protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active &#x3b1;4Gal-T1 protein as an expression system for recombinant production of such glycosphingolipids. Also a method for the identification of DNA sequence variations in the &#x3b1;4Gal-T1-coding exon by PCR, and detecting the presence of DNA sequence variation, are disclosed.

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Pandrup, Denmark

Rudi Steffensen