Burnham Institute for Medical Research

Sanford-Burnham Medical Research Institute

Ruchi Bajpai

Alexey Terskikh

Alexy Terskikh

We used ACCUTASE&#xae;, a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, ACCUTASE&#xae; treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with ACCUTASE&#xae; to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. ACCUTASE&#xae;-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency.

Methods for culture and production of single cell populations of human embryonic stem cells (HESCS)

Methods for culture and production of single cell populations of human embryonic stem cells

This invention provides pure populations of neural precursor cells, capable of differentiation into neurons, glial cells, and astrocytes. The populations are obtained by culturing stem cell populations (such as embryonic stem cells) in a cocktail of growth conditions that initiates differentiation, and establishes the neural precursor population. The precursors can be further differentiated in culture into a variety of different neural phenotypes. The neural precursors can be generated in pure form (at least 99%) and in large quantities for use in drug screening and the treatment of neurological disorders.

Homogeneous neural precursor cells

We used Accutase&#x2122;, a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, Accutase treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with Accutase to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. Accutase-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency.

Growing community of inventors

Redwood City, CA, United States of America

Ruchi Bajpai