Eastman-kodak Company

Royden N Rand

John C Mauck

Linda A Mauck

Charles A Goffe, Deceased

Robert Edwin Emmons

Tai W Wu

Angelo P Andrese

TaiWing Wu

Dry transparent analytical elements can be used to determine analytes which have been separated by electrically induced migration through a solid medium, e.g. by electrophoresis, or which are intracellular enzymes. The dry transparent element is placed on a plate containing the analytes, and kept there until the analytes have reacted to produce a non-diffusible detectable species solely in the element. The element is removed and the detectable species is evaluated therein. The elements contain a water-insoluble binder material having an interactive composition dispersed therein which reacts with analyte to produce the non-diffusible species. The same electrophoretic plate can be used to successively determine the same or a plurality of analytes since it is not altered or destroyed by contact with the dry element.

Use of dry analytical elements to determine analytes

Analytical element for the detection of an agent under analysis, termed analyte, dissolved in an aqueous, proteinaceous liquid. The element has at least two superposed layers, the layers including a spreading layer and a reagent layer. The element can include a support material which is preferably radiation transmissive. To normalize transport of the analyte in and through the spreading layer, the spreading layer contains an effective amount of a surfactant, such as a non-ionic surfactant. Preferably, the amount of surfactant is between about 1% and about 15% by weight of solid contents in the spreading layer.

Integral analytical element

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Pittsford, NY, United States of America

Royden N Rand