Eastman-kodak Company

Johnson & Johnson Clinical Diagnostics, Inc.

Roy Eugene Snoke

Charles T Goodhue

James R Schaeffer

Harry Wayne Harris

Hugh Arthur Risley

Theodore W Esders

Bruce E Babb

Gerald W Klein

Marsha D Oenick

Margaret Elizabeth Logan

Gary L Snodgrass

Gary M Underwood

Carol Anne DeCann

Modified apo-peroxidases have been found useful in analytical methods to remove the bias caused by the presence of hemoglobin in the test specimens. While apo-peroxidases are known to remove serum interferents, they fail to overcome the hemoglobin bias, particularly when dry coated analytical elements are used in the assays. Blocking the nitrogen atoms of the imidzolyl groups of the histidine amino acids of the apo-peroxidase prevents catalytic activity of the protein in the presence of hemoglobin.

Analytical, element, composition and method using modified

A dry immunoassay analytical element for assaying a ligand, comprising a support bearing:

Reduction in first slide bias and improved enzyme stability by the

New substrates for .gamma.-glutamyltransferase (.gamma.-GT) are disclosed. These subtrates are useful in the colorimetric determination of .gamma.-GT since the cleavage of the .gamma.-glutamyl group produces a detectable species--an aminostyrylquinolinium dye. The substrates have the formula: ##STR1## wherein: R and R.sub.1 are independently selected from hydrogen, alkyl and alkoxy and

Substrates, compositions, elements and methods for the determination of

A method for the purification and recovery of cholesterol oxidase is disclosed. Cholesterol oxidase is purified by a method including the steps of:

Method for the purification of cholesterol oxidase

A composition and element for assaying a neuraminic acid comprises a neuraminic acid aldolase, pyruvate oxidase and an electron acceptor. A sample containing a neuraminic acid can be contacted with the composition and the product detected spectrophotometrically.

Assay for neuraminic acids

A chromatographic material comprising an inorganic support-polysaccharide particle matrix is described. The matrix comprises an inorganic support having a high surface density of hydroxyl groups and, covalently attached thereto, insoluble particles of a polysaccharide. The free hydroxyl groups of the matrix can be activated, say, by treatment with cyanogen bromide or sodium metaperiodate, to form active sites where ligands can be attached for affinity chromatography.

Composition for chromatography

Catalase is effectively removed from a uricase preparation by amphiphilic chromotography using as the column material cyanogen bromide-activated polysaccharide having a hydrophobic ligand attached thereto. Particularly useful hydrophobic ligands are the alkanediamines.

Purification of uricase

A stable uricase preparation of high activity is produced by growing Micrococcus luteus NRRL B-8166 in a nutrient medium. Uricase preparations having activities of up to about 1000 U/liter are produced.

Production of uricase from micrococcus luteus

Nucleic acids and unwanted proteins are removed from microbial enzyme extracts by precipitation with water-soluble, cationic polymers comprised of monomers having the structure ##STR1## wherein R.sub.1 is a hydrogen atom, a lower alkyl group, or the equivalent and R.sub.2 is a nitrogen containing group capable of carrying a positive electrical charge when the polymer is dissolved in an aqueous solution.

Purification of microbial enzyme extracts using synthetic

An integral analytical element for analysis of liquids for their cholesterol content is disclosed. The element is of the type which comprises at least two superposed layers including a spreading layer and a reagent layer in fluid contact and, optionally, a support. Cholesterol oxidase and a composition for the hydrolysis of cholesterol esters comprising lipase having cholesterol esterase activity and protease are included in the element such that cholesterol esters contained in a sample applied to the spreading layer are saponified to free cholesterol and free cholesterol is decomposed in the presence of cholesterol oxidase to produce a detectable change related to the total cholesterol content of the sample.

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Webster, NY, United States of America

Roy Eugene Snoke