Hoffmann-la Roche Inc.

Roy Cleeland, Jr

Manfred Weigele

Willy Leimgruber

Emanuel Grunberg

Michael J Kramer

Hans Jacob Hager

Joanne Durkin

Compounds of the formula ##STR1## wherein R.sub.1 is lower alkyl or phenyl lower alkyl; R.sub.2 is phenyl or substituted phenyl; and R.sub.3 is substituted or unsubstituted phenyl, naphthyl or indolyl; are fluorogenic reagents which react with primary amino groups to form fluorophors. The above compounds are useful for fluorescent labeling of materials of biological importance.

Fluorogenic materials and labeling techniques

A novel labelled antigen consisting of .sup.125 I-rubella virus and its use in a radioimmunoassay for rubella virus specific antibodies in human serum is disclosed.

Radiolabelled rubella virus and method

Compounds of the formula ##STR1## wherein R.sub.1 is lower alkyl or phenyl lower alkyl; R.sub.2 is phenyl or substituted phenyl; and R.sub.3 is substituted or unsubstituted phenyl, naphthyl or indolyl; Are fluorogenic reagents which react with primary amino groups to form fluorophors. The above compounds are useful for fluorescent labeling of materials of biological importance.

1-Dimethylamino-2,4-diphenyl-1-butene-3,4-dione

Compounds of the formula ##STR1## wherein R.sub.1 is lower alkyl or phenyl lower alkyl: R.sub.2 is phenyl or substituted phenyl: and R.sub.3 is substituted or unsubstituted phenyl, naphthyl or indolyl; Are fluorogenic reagents which react with primary amino groups to form fluorophors. The above compounds are useful for fluorescent labeling of materials of biological importance.

Certain 2-hydroxy-2,4-diaryl-3-furanones

Compounds of the formula ##STR1## wherein R.sub.1 is lower alkyl or phenyl lower alkyl: R.sub.2 is phenyl or substituted phenyl; and R.sub.3 is substituted or unsubstituted phenyl, naphthyl or indolyl; Are fluorogenic reagents which react with primary amino groups to form fluorophors. The above compounds are useful for fluorescent labeling of materials of biological importance.

Amino lower alkyl ethers of the phenolic hydroxyl group of opium alkaloids are linked via a peptide linkage to carboxylated latex polymers to form reagents which are useful in a sensitive diagnostic test to detect the presence of opium alkaloids in body fluids.

Growing community of inventors

Short Hills, NJ, United States of America

Roy Cleeland, Jr