Merck + Co., Inc.

Roy A Machlowitz

William J McAleer

William J Miller

Henry Z Markus

Edward H Wasmuth

Alan C Herman

Influenza virus is extracted with a mixture of ether and ethanol, the aqueous and organic phases are separated and residual ether removed from the virus suspension under reduced pressure. The resulting product is useful as an influenza vaccine.

Influenza vaccine

Semi-automated assays for viral infectivity and an assay for serum neutralizing antibody content are based on staining the cells and macroscopic reading of the cytopathic effect.

Macroscopic method for determining cytopathic effects in viral assay

Automated tests to evaluate the antibody titer of a sample by incubating a test sample, bacteria, and complement, and detecting bacterial growth in the incubated mixture, and to identify bacteria and its titer in a sample by incubating a test sample, bacterial antiserum, and complement, and detecting inhibition of bacterial growth in the incubated mixture. The automated tests lend themselves to diagnostic use and may be provided in the form of diagnostic test kits.

Bacterial or bacterial antibody assay

Trachoma agent is separated from growth medium impurities by density gradient centrifugation in a sodium bromide solution.

Growing community of inventors

Glenside, PA, United States of America

Roy A Machlowitz