Roquette Freres

Roselyne Bernard

Pierre Lanos

Fabrice Allain

Agnès Denys

Héla Hacine-Gherbi

Mathieu Carpentier

Marc Biguet

Agnes Denys

Hela Hacine-Gherbi

The present invention relates to a biological method for the dosage of peptidoglycans in a sample, especially a sample of glucose polymers.

Biological dosage of peptidoglycans

The present invention relates to a biological method for assaying peptidoglycans (PGN) in a sample, particularly a sample of glucose polymers. The PGN assay includes: a) treating the glucose polymer sample by sonication, heating, and/or alkalizing; b) placing the treated sample or a dilution thereof in contact with a recombinant cell expressing an exogenous TLR2 (toll-like receptor 2) and a reporter gene directly dependent on the signaling pathway associated with the TLR2. The reporter gene codes for a colored or fluorescent protein or for a protein the activity of which is measurable with or without a substrate; c) measuring the reporter gene signal; and d) determining the amount of PGN in the sample using a standard curve of the correlation between the amount or PGN and the strength of the reporter gene signal.

Biological assay of peptidoglycans

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Lestrem, France

Roselyne Bernard