University of California

Lucky Biotech Corporation

Rosalind Kim

Sung-Hou Kim

Robert L Fischer

Joong M Cho

James Giovannoni

Lola Penarrubia

Jamila Jancarik

An optimum solubility screen in which a panel of buffers and many additives are provided in order to obtain the most homogeneous and monodisperse protein condition for protein crystallization. The present methods are useful for proteins that aggregate and cannot be concentrated prior to setting up crystallization screens. A high-throughput method using the hanging-drop method and vapor diffusion equilibrium and a panel of twenty-four buffers is further provided. Using the present methods, 14 poorly behaving proteins have been screened, resulting in 11 of the proteins having highly improved dynamic light scattering results allowing concentration of the proteins, and 9 were crystallized.

High-throughput method for optimum solubility screening for homogeneity and crystallization of proteins

Edible fruit, seed and vegetables of transgenic plants modified to produce a sweetening protein such as monellin or thaumatin are useful in preparing food compositions which have enhanced sweetness improved flavor. Expression systems for the genes encoding sweetening proteins compatible with plant systems and designed to enhance the production of these proteins in the edible portions of plants, and methods for producing sweetened fruit, seeds and vegetables are described.

Endogenously sweetened transgenic plant products

Constructs for expression of monellin in plant cells

Growing community of inventors

Moraga, CA, United States of America

Rosalind Kim