Cibus US LLC

Cibus Europe, B.v.

Rosa E Segami

Peter R Beetham

Gregory F W Gocal

James Pearce

Christian Schopke

Noel Sauer

Jerry Mozoruk

Noel Joy Sauer

Gregory FW Gocal

The invention provides to improved methods for the modification of genes in plant cells, and plants and seeds derived therefrom. More specifically, the invention relates to the increased efficiency of targeted gene mutation by combining gene repair oligonucleotides with approaches that enhance the availability of components of the target cell gene repair mechanisms.

Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair

Provided herein include methods and compositions for effecting a targeted genetic change in DNA in a cell. Certain aspects and embodiments relate to improving the efficiency of the targeting of modifications to specific locations in genomic or other nucleotide sequences. As described herein, nucleic acids which direct specific changes to the genome may be combined with various approaches to enhance the availability of components of the natural repair systems present in the cells being targeted for modification.

Provided herein include methods and compositions for making targeted changes to a DNA sequence. In various aspects and embodiments, methods and compositions for modifying a DNA sequence in a cell (such as a plant, bacterial, yeast, fungal, algal, or mammalian cell) are provided. In some aspects and embodiments the modification of DNA involves combining gene repair oligonucleotides with approaches that enhance the availability of components of the target cell gene repair mechanisms, such as a DNA cutter.

Targeted gene modification using oligonucleotide-mediated gene repair

Methods and compositions for targeted gene modification

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Escondido, CA, United States of America

Rosa E Segami