Danisco US Inc.

The Procter Gamble Company

Roopa Santosh Ghirnikar

Frits Goedegebuur

Lilia Maria Babe

Jian Yao

David Aaron Estell

Richard Ray Bott

Rie Mejldal

Marc Anton Bernhard Kolkman

Katherine Augustyn

Philip Frank Souter

Marc Kolkman

Thijs Kaper

Sina Pricelius

Eva Maria Perez-Prat Vinuesa

Harm Jan Mulder

Carly Pickering

Viktor Alekseyev

Sander Van Stigt Thans

Michelle Jackson

Xiaogang Gu

Lydia Dankmeyer

Michael Stoner

Adam Garske

Christian D Adams

Nils Henning Redestig

Viktor Yuryevich Alekseyev

Liling Jin

Zhen Qian

Shukun Yu

Victoria Huang

Harm Mulder

The present disclosure provides AprL-clade protease enzymes, including variant AprL-clade protease enzymes, nucleic acids encoding same, and compositions and methods related to the production and use thereof, including an AprL-clade variant subtilisin enzyme that has improved stability and/or soil removal compared to a parent AprL-clade subtilisin enzyme.

AprL-clade protease variants and uses thereof

A phosphate-free automatic dishwashing cleaning composition comprising a protease wherein the protease has at least 80%, preferably at least 85%, more preferably at least 90% and especially at least 96% identity with the amino acid sequence of SEQ ID NO:1 or with the amino acid sequence of SEQ ID NO:2 and wherein the protease comprises amino acid substitutions selected from the group consisting of:

Automatic dishwashing detergent composition comprising a protease

AprL-clade subtilisin protease variants and uses thereof

Aspects of the present compositions and methods relate to novel metalloproteases, polynucleotides encoding the novel metalloproteases, and compositions and methods for use thereof.

Metalloproteases

Growing community of inventors

Sunnyvale, CA, United States of America

Roopa Santosh Ghirnikar