Dade International Inc.

Weyerhaeuser Company Limited

Cetus Corporation

Weyerhaeuser

Rony Tal

Hing C Wong

Moshe Benziman

David H Gelfand

Arie Ben-Bassat

Pierre-Andre Chavaillaz

Clayton Casipit

Vaughan Wittman

Roger D Calhoon

Anna Lisa Fear

James H Meade

The present invention provides novel methods for the synthesis and isolation and purification of a peptide of interest (target peptide). In particular, the invention relates to peptide synthesis, isolation and purification methods that comprise use of penI fusion polypeptides and related gene fusion constructs that encode such polypeptides.

Peptide synthesis and purification by fusion to penI protein or

Methods for peptide synthesis and purification

The present invention provides the nucleotide sequences of Acetobacter operons, cdg operons encoding genes for the biosynthesis and degradation of cyclic diguanosine monophosphate (c-di-GMP). Specifically, the nucleotide sequences and deduced amino acid sequences of 3 phosphodiesterases isozymes, 3 diguanylate cyclase isozymes, and 2 polypeptides of unidentified function are provided. Also provided for are various strains of microorganisms, including Acetobacter cells genetically manipulated so as to produce elevated and/or reduced levels of one or more cdg operon encoded proteins.

Cyclic di-guanylate metabolic enzymes

Novel proteins capable of binding to bacterial cellulose synthase are provided. Proteins that co-purify with bacterial cellulose synthase and have approximate relative molecular weights of 20 kDa, 54 kDa and 59 kDa are taught. Also provided for are nucleotide sequences encoding the proteins, antibodies to the proteins, and recombinant host cells for the expression of the cellulose synthase associated (co-purifying) proteins. The cellulose synthase associated proteins (CSAPs) CSAP20 (SEQ ID NO: 1) CSAP54 (SEQ ID NO: 2), and CSAP59 (SEQ ID NO: 3) are specifically provided for.

Cellulose synthase associated proteins

Nucleic acid sequences encoding the bacterial cellulose synthase operon derived from Acetobacter are disclosed. Methods for isolating the genes, vectors containing the genes, and transformed hosts useful for the expression of recombinant bacterial cellulose synthase or production of cellulose are also described.

Methods and nucleic acid sequences for the expression of the cellulose

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Coral Springs, FL, United States of America

Rony Tal