Sanofi

Ronnie Wei

Gary J Nabel

Joerg Thomas Regula

Huawei Qiu

Ercole Rao

Béatrice Cameron

Tarik Dabdoubi

Christian Beil

Cendrine Lemoine

Catherine Prades

Ling Xu

Zhi-Yong Yang

Lan Wu

Jochen Beninga

Edward Seung

Joerg Birkenfeld

Zhen Xing

Provided herein are trispecific and/or trivalent binding proteins comprising four polypeptide chains that form three antigen binding sites that specifically bind one or more target proteins, wherein a first pair of polypeptides forming the binding protein possess dual variable domains having a cross-over orientation, and wherein and a second pair of polypeptides possess a single variable domain forming a single antigen binding site. In some embodiments, the binding proteins comprise a binding site that binds a CD28 polypeptide, a binding site that binds a CD3 polypeptide, and a binding site that binds a third polypeptide, such as a tumor target protein. In some embodiments, the binding proteins comprise four polypeptide chains that form three antigen binding sites that specifically bind one or more HIV target proteins. The disclosure also relates to methods for making trispecific and/or trivalent binding proteins and uses of such binding proteins.

Trispecific binding proteins, methods, and uses thereof

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Needman, MA, United States of America

Ronnie Wei