Ge Healthcare Bio-sciences Ab

Cytiva Bioprocess R&d Ab

Ge Healthcare Bioprocess R&d Ab

Cytiva Sweden Ab

Ge Healthcare UK Limited

Ronnie Palmgren

Jean-Luc Maloisel

Mattias Algotsson

Nils Norrman

Ola Lind

Bengt Bjellqvist

Gustav Jose Rodrigo

Lars C Andersson

Bjorn Noren

Philippe Busson

Eggert Brekkan

Jonas Bengtsson

Klaus Gebauer

Tomas Bjorkman

Mats Ander

Robert Scott Youngquist

Anders Larsson

Jimmy Hedin Dahlstrom

Lee Hedemyr

Andreas Axen

Hans J Johansson

Jinyu Zou

James Van Alstine

Eric Faldt

Andreas Torbjorn Lundin

Johan William Ohman

Erik Bjerneld

Johan Fredrik Avallin

Per Erik Emilsson

Barry Johnson

Karl Axel Jakob Liderfelt

Eva Holmgren

Thomas Woods Keough

Ylva Laurin

Karl Liderfelt

Elisabeth Hallgren

Linn Carlsson

Maria Liminga

Lena Stigh

Tomas Björkman

Jesper Hedberg

Niklas Jungnelius

Anita Solbrand

Johan Avallin

Ingmar Olsson

Michael Morrison

Linda Svensson

Jan-Olof Johansson

John Daicic

Klas Allmer

Sarah Janet Clegg

Douglas Mark Green

Philip Robert Mallender

Miles W Burrows

Fernando Polanco

Maranda Fordham

Jimmy Hedin Dahlström

Charlotte Brink

Sofia Söderberg

Barry Edmund Johnson

The invention discloses a separation matrix comprising polysaccharide gel beads, wherein said polysaccharide gel beads comprise embedded fibers. The invention further discloses a method of preparing the separation matrix and use of the matrix for separation purposes.

Separation matrix and method of separation

The invention discloses an immunoglobulin-binding magnetic bead, comprising a porous matrix and one or more magnetic particles embedded in said matrix, wherein said matrix comprises a porous polymer and at least 10 mg/ml Fc-binding proteinaceous ligands covalently coupled to said porous polymer.

Magnetic immunoglobulin-binding particles

A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: &#x2014;providing a feed from a cell culture () comprising said biomolecule to a magnetic separator () and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; &#x2014;separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; &#x2014;forwarding said magnetic beads as a slurry with an added buffer to an elution cell (); &#x2014;eluting the bound biomolecules in the elution cell.

Method and system for separating biomolecules

Methods that include providing and reacting a solid support and an alkali-stable ligand derived from an immunoglobulin-binding bacterial protein to form a separation matrix having covalently coupled alkali-stable ligands; and washing with a wash solution comprising at least 10 mM of an alkali metal hydroxide.

Method for preparation of a separation matrix

The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.

Chromatography method

A system and method for producing fine droplets of polysaccharide from a premixed water-in-oil emulsion uses a packed bed () comprising hydrophilic beads ().

Packed bed emulsification

The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.

Chelator and use thereof

The invention discloses a cellulose or glass fiber paper for preservation of biological samples, which comprises 4-30 wt % of a hydrophilic branched carbohydrate polymer. It also discloses a method for preservation of biological samples by applying and drying them on the paper.

Sample preservation method and sample preservation substrate

The invention discloses a sample preservation method which comprises the following steps: a) providing a paper substrate comprising ligands, where the ligands comprise charged groups, b) applying a sample comprising at least one analyte and at least one contaminant on the paper substrate, c) drying the sample on the paper substrate and, d) extracting at least part of the paper substrate to provide a solution of the analyte.

Sample preservation method and sample application substrate

The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity. The dimeric pentadentate chelator according to the invention has the following general formula:  

The invention relates to a system and method for the stable storage of sensitive biological or chemical target substance, in a bound form on certain capture media. The method comprised providing a sample containing the target substance in a suitable buffer; combining the sample with a capture media to effect reversible binding of the target substance to the capture media; and storing the capture media with the target substance at between about &#x2212;20 and 20&#xb0; C.; and recovering the target substance from the capture media. The target substance recovered maintains the desired activity. Also provides are methods for reducing aggregates in the sensitive biological or chemical target substance.

Method for storage and stabilization of a target substance

The invention discloses a polypeptide capable of binding immunoglobulins or immunoglobulin-containing proteins, which polypeptide comprises six or more domains of protein Z or the C domain of protein A or a functional variant thereof. It also discloses separation matrices comprising the polypeptide and methods of using the separation matrices for separation of immunoglobulins or immunoglobulin-containing proteins.

Affinity chromatography matrix

The invention relates to a method to produce gels for electrophoresis, wherein at least two monomer solutions are printed in a pattern on a substrate and the printed substrate is exposed to electromagnetic or ionising radiation so as to initiate polymerisation. The gels are useful for electrophoretic separation of proteins, peptides and/or nucleic acids.

Printing of electrophoresis gels

The present invention relates to a device and method for separation of proteins and other biomolecules. A preferred use is for sample preparation of crude as well as pre-fractionated samples. In a preferred embodiment, the device of the present invention is a pipette tip having dual channels, one for inlet of sample and one for outlet. The outlet, but not the inlet, channel is provided with sample separation media for separation of a desired biomolecule from a sample. The flow through the sample separation media is unidirectional.

Device and method for separation of proteins and other biomolecules

Growing community of inventors

Uppsala, Sweden

Ronnie Palmgren