Danisco US Inc.

Ronaldus W J Hommes

David Aaron Estell

Andrew Shaw

Hiroshi Oh

Wolfgang Aehle

Louise Wallace

Ayrookaran J Poulose

Eugene Steven Sadlowski

Brian E Jones

Marc Kolkman

Chris Leeflang

Sang-Kyu Lee

Wilhelmus A H Van Der Kley

Leonardus P M Van Marrewijk

Amy D Liu

Richard Ray Bott

Frits Goedegebuur

Benjamin S Bower

Bradley R Kelemen

Thijs Kaper

Slavko Kralj

Igor Nikolaev

Gudrun Vogtentanz

Mats Sandgren

Sander Van Stigt Thans

Jonathan Caspi

Johan Van Lieshout

The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or catalytic activity. In some embodiments, the thermolysin finds use in cleaning and other applications comprising detergent. In some particularly preferred embodiments, the present invention provides methods and compositions comprising thermolysin formulated and/or engineered to resist detergent-induced inactivation.

Thermolysin variants

The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained fromsp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from. In still further preferred embodiments, the neutral metalloprotease is a variant of theneutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the theneutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

Use and production of storage-stable neutral metalloprotease

The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.

Multiple mutation variants of serine protease

The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.

Cellulase variants with improved expression, activity and stability, and use thereof

The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained fromsp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from. In still further preferred embodiments, the neutral metalloprotease is a variant of theneutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of theneutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

Thermostable neutral metalloproteases

Thermolysin variants and detergent compositions therewith

The present invention provides novelspp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.

Cellulomonas 69B4 serine protease variants

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Haarlem, Netherlands

Ronaldus W J Hommes